Invitrogen

CD38 Monoclonal Antibody (90), PerCP-eFluor™ 710, eBioscience™

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CD38 Antibody (46-0381-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.03 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.03 µg of Anti-Mouse CD38 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-0381-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06 µg/test

查看17篇已发表文章 17篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10853677

产品详细信息

Description: The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.

Applications Reported: This 90 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD38 (NAD+ glycohydrolase) is a type II transmembrane glycoprotein able to induce activation, proliferation and differentiation of mature lymphocytes and mediate apoptosis of myeloid and lymphoid progenitor cells. CD38 functions as a multi-catalytic ectoenzyme serving as ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase and possibly NAD+ glycohydrolase or as a cell surface receptor. Antibodies to CD38 are useful in subtyping of lymphomas and leukemias, detection of plasma cells (i.e. identification of myelomas), and as a marker for activated B and T cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. Further, CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma and neuroblastoma. Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter's Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Establishment and Characterization of Free-Floating 3D Macrophage Programming Model in the Presence of Cancer Cell Spheroids.

International journal of molecular sciences

Korotkaja K,Jansons J,Spunde K,Rudevica Z,Zajakina A

46-0381-82 was used in Flow cytometry/Cell sorting to establish and characterized a new in vitro 3D model for macrophage programming in the presence of cancer cell spheroids.

Wed Jun 28 00:00:00 UTC 2023

Establishment and Characterization of Free-Floating 3D Macrophage Programming Model in the Presence of Cancer Cell Spheroids.

International journal of molecular sciences

Korotkaja K,Jansons J,Spunde K,Rudevica Z,Zajakina A

46-0381-82 was used in Flow cytometry/Cell sorting to establish and characterized a new in vitro 3D model for macrophage programming in the presence of cancer cell spheroids.

Wed Jun 28 00:00:00 UTC 2023

Gammaherpesvirus-mediated repression reveals EWSR1 to be a negative regulator of B cell responses.

Proceedings of the National Academy of Sciences of the United States of America

Wang Y,Feswick A,Apostolou V,Petkov PM,Moser EK,Tibbetts SA

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Tue Aug 09 00:00:00 UTC 2022

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Thu Jan 07 00:00:00 UTC 2021

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2021

Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7).

EMBO molecular medicine

Bernasconi R,Thriene K,Romero-Fernández E,Gretzmeier C,Kühl T,Maler M,Nauroy P,Kleiser S,Rühl-Muth AC,Stumpe M,Kiritsi D,Martin SF,Hinz B,Bruckner-Tuderman L,Dengjel J,Nyström A

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Thu Oct 07 00:00:00 UTC 2021

CD11b Deficiency Exacerbates Methicillin-Resistant Staphylococcus aureus-Induced Sepsis by Upregulating Inflammatory Responses of Macrophages.

Immune network

Sim H,Jeong D,Kim HI,Pak S,Thapa B,Kwon HJ,Lee K

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Cancers

Nasarre P,Garcia DI,Siegel JB,Bonilla IV,Mukherjee R,Hilliard E,Chakraborty P,Nasarre C,Yustein JT,Lang M,Jaffa AA,Mehrotra S,Klauber-DeMore N

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Sun May 30 00:00:00 UTC 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Wed Oct 27 00:00:00 UTC 2021

CHK1 dosage in germinal center B cells controls humoral immunity.

Cell death and differentiation

Schoeler K,Jakic B,Heppke J,Soratroi C,Aufschnaiter A,Hermann-Kleiter N,Villunger A,Labi V

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Sun Dec 01 00:00:00 UTC 2019

DC3 is a method for deconvolution and coupled clustering from bulk and single-cell genomics data.

Nature communications

Zeng W,Chen X,Duren Z,Wang Y,Jiang R,Wong WH

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Thu Oct 10 00:00:00 UTC 2019

B Cell Receptor Crosslinking Augments Germinal Center B Cell Selection when T Cell Help Is Limiting.

Cell reports

Turner JS,Ke F,Grigorova IL

46-0381-82 was used in Flow cytometry/Cell sorting to investigate how T cells influence germinal center B cell expansion and plasmablast formation.

Tue Nov 06 00:00:00 UTC 2018

Antigen Acquisition Enables Newly Arriving B Cells To Enter Ongoing Immunization-Induced Germinal Centers.

Journal of immunology (Baltimore, Md. : 1950)

Turner JS,Benet ZL,Grigorova IL

46-0381 was used in Flow cytometry/Cell sorting to show that naive B cells have a limited window of time during which they can undergo Ag-driven activation and join ongoing immunization-induced germinal center responses.

Tue Aug 15 00:00:00 UTC 2017

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Thu Oct 12 00:00:00 UTC 2017

Artery Tertiary Lymphoid Organs Control Multilayered Territorialized Atherosclerosis B-Cell Responses in Aged ApoE-/- Mice.

Arteriosclerosis, thrombosis, and vascular biology

Srikakulapu P,Hu D,Yin C,Mohanta SK,Bontha SV,Peng L,Beer M,Weber C,McNamara CA,Grassia G,Maffia P,Manz RA,Habenicht AJ

46-0381 was used in Flow cytometry/Cell sorting to study how artery tertiary lymphoid organs can orchestrate B-cell responses in the diseased aorta.

Wed Jun 01 00:00:00 UTC 2016

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Tue Sep 06 00:00:00 UTC 2016

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Wed Sep 16 00:00:00 UTC 2015

A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.

The Journal of clinical investigation

Dai X,James RG,Habib T,Singh S,Jackson S,Khim S,Moon RT,Liggitt D,Wolf-Yadlin A,Buckner JH,Rawlings DJ

Published figure using CD38 monoclonal antibody (Product # 46-0381-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2013

生物信息学

蛋白别名: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen p45; Cyclic ADP-ribose hydrolase 1; I-19; NAD+ nucleosidase; NIM-R5 antigen

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基因别名: ADPRC 1; Cd38; Cd38-rs1

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UniProt ID: (Mouse) P56528

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Entrez Gene ID: (Mouse) 12494

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功能

NAD+ nucleosidase activity transferase activity hydrolase activity hydrolase activity, acting on glycosyl bonds phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

参与通路