Invitrogen

CD42b Monoclonal Antibody (HIP1), PE, eBioscience™

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CD42b Antibody (12-0429-42) in Flow

Staining of human platelets with Anti-Human CD41a FITC (Product # 11-0419-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD42b PE (right). Total events were used for analysis.

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产品信息

12-0429-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

查看5篇已发表文章 5篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HIP1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10852864

产品详细信息

Description: The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.

Applications Reported: The HIP1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIP1 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. CD42b expression and activation seems to be regulated by VEGF and PDGF. CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Small-Molecule Cyclophilin Inhibitors Potently Reduce Platelet Procoagulant Activity.

International journal of molecular sciences

Van Bael J,Vandenbulcke A,Ahmed-Belkacem A,Guichou JF,Pawlotsky JM,Samyn J,Barendrecht AD,Maas C,De Meyer SF,Vanhoorelbeke K,Tersteeg C

Published figure using CD42b monoclonal antibody (Product # 12-0429-42) in Flow Cytometry

Wed Apr 12 00:00:00 UTC 2023

A cluster of immunoresolvents links coagulation to innate host defense in human blood.

Science signaling

Norris PC,Libreros S,Chiang N,Serhan CN

12-0429 was used in Flow cytometry/Cell sorting to explore the relationship between coagulation and the resolution of inflammation and infection by lipid mediators through metabololipidomics-based profiling of human whole blood during coagulation.

Tue Aug 01 00:00:00 UTC 2017

Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways.

Genome biology

Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I

12-0429 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity.

Tue May 03 00:00:00 UTC 2016

Platelet-derived HMGB1 is a critical mediator of thrombosis.

The Journal of clinical investigation

Vogel S,Bodenstein R,Chen Q,Feil S,Feil R,Rheinlaender J,Schäffer TE,Bohn E,Frick JS,Borst O,Münzer P,Walker B,Markel J,Csanyi G,Pagano PJ,Loughran P,Jessup ME,Watkins SC,Bullock GC,Sperry JL,Zuckerbraun BS,Billiar TR,Lotze MT,Gawaz M,Neal MD

Published figure using CD42b monoclonal antibody (Product # 12-0429-42) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2015

Identification of inherited macrothrombocytopenias based on mean platelet volume among patients diagnosed with idiopathic thrombocytopenia.

Thrombosis research

Gohda F,Uchiumi H,Handa H,Matsushima T,Tsukamoto N,Morita K,Amagai H,Murakami M,Murakami H,Nojima Y,Karasawa M

Thu Jun 14 00:00:00 UTC 2007

Staphylococcus aureus adhesion via Spa, ClfA, and SdrCDE to immobilized platelets demonstrates shear-dependent behavior.

Arteriosclerosis, thrombosis, and vascular biology

George NP,Wei Q,Shin PK,Konstantopoulos K,Ross JM

Sun Oct 01 00:00:00 UTC 2006

生物信息学

蛋白别名: Antigen CD42b-alpha; CD42a; CD42b; glycoprotein Ib (platelet), alpha polypeptide; GP-Ib alpha; GP-IX; GPIB; GPIb alpha; GPIX; MGC34595; mutant platelet membrane glycoprotein Ib-alpha; Platelet glycoprotein Ib alpha chain; Platelet GPIX; platelet membrane glycoprotein 1b-alpha subunit; platelet membrane glycoprotein Ib-alpha

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基因别名: BDPLT1; BDPLT3; BSS; CD42B; CD42b-alpha; DBPLT3; GP1B; GP1BA; GPIbA; GPIbalpha; VWDP

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UniProt ID: (Human) P07359

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Entrez Gene ID: (Human) 2811

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功能

protein kinase inhibitor activity protein binding thrombin receptor activity

参与通路