Invitrogen

CD45 Monoclonal Antibody (OX1), APC, eBioscience™

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CD45 Antibody (17-0461-82) in Flow

Staining of rat splenocytes with 0.25 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or 0.25 µg of Anti-Rat CD45 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

17-0461-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Rat

已发表种属

Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

OX1

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10717666

产品详细信息

Description: This OX1 monoclonal antibody recognizes a non-polymorphic determinant of rat CD45, which is also known as the leukocyte common antigen. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. This type I transmembrane protein tyrosine phosphatase exists as a variety of different isoforms due to alternative splicing.

Applications Reported: This OX1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX1 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

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17-0461-82 was used in Flow Cytometry to show that plantar fascia-derived cells (PFCs) expressed common MSC surface markers and embryonic stemness markers.

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Frontiers in physiology

Sharma GP,Frei A,Fish B,Gasperetti T,Veley D,Szalewski N,Nissen A,Himburg HA

17-0461-82 was used in Flow cytometry/Cell sorting to suggest a role for ACE-expressing myeloid cells in the pathogenesis of radiation pneumonitis.

Wed Jun 07 00:00:00 UTC 2023

Biological sex differences in renin angiotensin system enzymes ACE and ACE2 regulate normal tissue response to radiation injury.

Frontiers in physiology

Sharma GP,Frei A,Fish B,Gasperetti T,Veley D,Szalewski N,Nissen A,Himburg HA

17-0461-82 was used in Flow cytometry/Cell sorting to suggest a role for ACE-expressing myeloid cells in the pathogenesis of radiation pneumonitis.

Wed Jun 07 00:00:00 UTC 2023

Propofol suppresses adipose-derived stem cell progression via PI3K/AKT-Wnt signaling pathway.

BMC anesthesiology

Yin G,Wang J,Zhong Y,Wu W

17-0461-82 was used in Flow Cytometry to indicate that the PI3K/AKT-Wnt pathways mediated propofol-inhibited ADSC proliferation, providing new insights into the propofol application in ADSCs.

Wed Mar 09 00:00:00 UTC 2022

Mitigation of Multi-Organ Radiation Injury with ACE2 Agonist Diminazene Aceturate.

Radiation research

Gasperetti T,Sharma GP,Frei AC,Pierce L,Veley D,Szalewski N,Narayanan J,Fish BL,Himburg HA

17-0461-82 was used in Flow cytometry/Cell sorting to suggest the ACE2 enzyme plays a critical role in the RAS-mediated pathogenesis of radiation injury and may be a potential therapeutic target for the development of medical countermeasures for acute radiation exposure.

Sat Oct 01 00:00:00 UTC 2022

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Journal of neuroinflammation

Kitase Y,Chin EM,Ramachandra S,Burkhardt C,Madurai NK,Lenz C,Hoon AH,Robinson S,Jantzie LL

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Tue Oct 19 00:00:00 UTC 2021

Neuron-Derived Extracellular Vesicles Modulate Microglia Activation and Function.

Biology

Peng H,Harvey BT,Richards CI,Nixon K

17-0461-82 was used in Flow Cytometry to support that neurons critically regulate microglia activity and control inflammation via EV-mediated neuron-glia communication.

Wed Sep 22 00:00:00 UTC 2021

Bifidobacteria shape host neural circuits during postnatal development by promoting synapse formation and microglial function.

Scientific reports

Luck B,Engevik MA,Ganesh BP,Lackey EP,Lin T,Balderas M,Major A,Runge J,Luna RA,Sillitoe RV,Versalovic J

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Subcutaneous administration of a neutralizing IL-1β antibody prolongs limb allograft survival.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

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17-0461 was used in Flow cytometry/Cell sorting to study the effect of intragraft treatment with a neutralisin IL-1B antibody in limb transplantation.

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Hypoxic preconditioned bone mesenchymal stem cells ameliorate spinal cord injury in rats via improved survival and migration.

International journal of molecular medicine

Wang W,Huang X,Lin W,Qiu Y,He Y,Yu J,Xi Y,Ye X

17-0461-82 was used in Flow Cytometry to suggest that HP may increase the survival and migratory abilities of BMSCs and highlights their therapeutic potential for SCI.

Thu Nov 01 00:00:00 UTC 2018

BMP14 induces tenogenic differentiation of bone marrow mesenchymal stem cells in vitro.

Experimental and therapeutic medicine

Wang D,Jiang X,Lu A,Tu M,Huang W,Huang P

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Combined treatment with simvastatin and rapamycin attenuates cardiac allograft rejection through the regulation of T helper 17 and regulatory T cells.

Experimental and therapeutic medicine

Liu Y,Sun L,Chen W,Chuai J,Shang Y,Zhang D,Fu B,Tian H,Jiang S

17-0461 was used in Flow cytometry/Cell sorting to study the benefits of combined simvastatin and rapamycin therapy for reducing rejection following cardiac transplant.

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CD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity in vitro.

Molecular medicine reports

Wu YX,Jing XZ,Sun Y,Ye YP,Guo JC,Huang JM,Xiang W,Zhang JM,Guo FJ

Published figure using CD45 monoclonal antibody (Product # 17-0461-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats.

Stem cell research & therapy

Yang H,Wu S,Feng R,Huang J,Liu L,Liu F,Chen Y

Published figure using CD45 monoclonal antibody (Product # 17-0461-82) in Flow Cytometry

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Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens.

International journal of clinical and experimental pathology

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17-0461-82 was used in Flow Cytometry to contribute to recruitment of BMSCs in injured area and deposition of collagens.

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Fibroblast Growth Factor-10 (FGF-10) Mobilizes Lung-resident Mesenchymal Stem Cells and Protects Against Acute Lung Injury.

Scientific reports

Tong L,Zhou J,Rong L,Seeley EJ,Pan J,Zhu X,Liu J,Wang Q,Tang X,Qu J,Bai C,Song Y

17-0461 was used in Flow cytometry/Cell sorting to reveal that the protective effect of FGF-10 might be mediated by the mobilisation of LR-MSCs in lungs.

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Leucine-rich repeat kinase 2 deficiency is protective in rhabdomyolysis-induced kidney injury.

Human molecular genetics

Boddu R,Hull TD,Bolisetty S,Hu X,Moehle MS,Daher JP,Kamal AI,Joseph R,George JF,Agarwal A,Curtis LM,West AB

17-0461 was used in Flow cytometry/Cell sorting to highlight several novel physiological roles for leucine-rich repeat kinase 2 in disposition to kidney disease.

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PRC2 inhibition counteracts the culture-associated loss of engraftment potential of human cord blood-derived hematopoietic stem and progenitor cells.

Scientific reports

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生物信息学

蛋白别名: CD45; L-CA; leucocyte common antigen; Leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; Protein tyrosine phosphatase, receptor-type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T220 and B220

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基因别名: CD45; L-CA; Lca; Ptprc; RT7; T200

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UniProt ID: (Rat) P04157

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Entrez Gene ID: (Rat) 24699

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功能