Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Functional Grade, eBioscience™

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)

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CD61 (Integrin beta 3) Antibody (16-0611-82) in Flow

Staining of mouse bone marrow with Anti-Mouse/Rat CD61 (Integrin beta 3) FITC (left) or PE (right). Appropriate isotype controls were used (open histogram). Cells in the myeloid population were used for analysis.

CD61 (Integrin beta 3) Antibody in Immunocytochemistry (ICC/IF)

CD61 (Integrin beta 3) Antibody in Immunohistochemistry (IHC)

CD61 (Integrin beta 3) Antibody in Western Blot (WB)

CD61 (Integrin beta 3) Antibody in ELISA, Neutralization (ELISA, Neu)

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产品信息

16-0611-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看8篇已发表文章 8篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2C9.G3

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_468984

产品详细信息

Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.

Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes.

Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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16-0611-82 was used in Immunohistochemistry to investigate what molecular contexts determine retinal ganglion cells susceptibility.

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A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

Molecular therapy oncolytics

Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

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Hoxa9 and Meis1 Cooperatively Induce Addiction to Syk Signaling by Suppressing miR-146a in Acute Myeloid Leukemia.

Cancer cell

Mohr S,Doebele C,Comoglio F,Berg T,Beck J,Bohnenberger H,Alexe G,Corso J,Ströbel P,Wachter A,Beissbarth T,Schnütgen F,Cremer A,Haetscher N,Göllner S,Rouhi A,Palmqvist L,Rieger MA,Schroeder T,Bönig H,Müller-Tidow C,Kuchenbauer F,Schütz E,Green AR,Urlaub H,Stegmaier K,Humphries RK,Serve H,Oellerich T

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

Mon Apr 10 00:00:00 UTC 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

Mon Jan 16 00:00:00 UTC 2017

The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes.

eLife

Benito-Jardón M,Klapproth S,Gimeno-LLuch I,Petzold T,Bharadwaj M,Müller DJ,Zuchtriegel G,Reichel CA,Costell M

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

Mon Jan 16 00:00:00 UTC 2017

Mammary-Stem-Cell-Based Somatic Mouse Models Reveal Breast Cancer Drivers Causing Cell Fate Dysregulation.

Cell reports

Zhang Z,Christin JR,Wang C,Ge K,Oktay MH,Guo W

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

Tue Sep 20 00:00:00 UTC 2016

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Tue Jun 28 00:00:00 UTC 2016

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Tue Jun 28 00:00:00 UTC 2016

WISP1-αvβ3 integrin signaling positively regulates TLR-triggered inflammation response in sepsis induced lung injury.

Scientific reports

Chen Z,Ding X,Jin S,Pitt B,Zhang L,Billiar T,Li Q

Tue Jun 28 00:00:00 UTC 2016

H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets.

Journal of hematology & oncology

Liu HD,Zhang AJ,Xu JJ,Chen Y,Zhu YC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

Wed Feb 24 00:00:00 UTC 2016

H2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets.

Journal of hematology & oncology

Liu HD,Zhang AJ,Xu JJ,Chen Y,Zhu YC

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

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Integrin-modulating therapy prevents fibrosis and autoimmunity in mouse models of scleroderma.

Nature

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Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

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Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Immunofluorescence

Mon Dec 08 00:00:00 UTC 2014

β1 integrins with individually disrupted cytoplasmic NPxY motifs are embryonic lethal but partially active in the epidermis.

The Journal of investigative dermatology

Meves A,Stremmel C,Böttcher RT,Fässler R

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Sun Dec 01 00:00:00 UTC 2013

Differentiation generates paracrine cell pairs that maintain basaloid mouse mammary tumors: proof of concept.

PloS one

Kim S,Goel S,Alexander CM

Published figure using CD61 (Integrin beta 3) monoclonal antibody (Product # 16-0611-82) in Flow Cytometry

Tue Apr 26 00:00:00 UTC 2011

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Fri Jul 31 00:00:00 UTC 2009

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Fri Jul 31 00:00:00 UTC 2009

Plasminogen activator inhibitor-1 regulates integrin alphavbeta3 expression and autocrine transforming growth factor beta signaling.

The Journal of biological chemistry

Pedroja BS,Kang LE,Imas AO,Carmeliet P,Bernstein AM

Fri Jul 31 00:00:00 UTC 2009

Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes.

Journal of immunology (Baltimore, Md. : 1950)

Nohara K,Pan X,Tsukumo S,Hida A,Ito T,Nagai H,Inouye K,Motohashi H,Yamamoto M,Fujii-Kuriyama Y,Tohyama C

Tue Mar 01 00:00:00 UTC 2005

生物信息学

蛋白别名: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

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基因别名: CD61; GP3A; INGRB3; Itgb3

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UniProt ID: (Rat) Q8R2H2, (Mouse) O54890

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Entrez Gene ID: (Rat) 29302, (Mouse) 16416

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功能

fibronectin binding protease binding protein disulfide isomerase activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding receptor activity integrin

参与通路

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Functional Grade, eBioscience™

CD61 (Integrin beta 3) Antibody (16-0611-82) in Flow

产品信息

16-0611-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

中和实验 (Neu)

功能检测 (Functional)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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