Invitrogen

CD63 Polyclonal Antibody

CD63 Antibody in Immunocytochemistry (ICC/IF)

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CD63 Antibody (PA5-92370) in ICC/IF

Immunofluorescence analysis of CD63 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD63 Polyclonal Antibody (Product # PA5-92370) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More

CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD63 Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of the target protein. U-87 MG cells were transfected with CD63 siRNA and a decrease in signal intensity was observed in ICC application using Anti-CD63 Polyclonal Antibody (Product # PA5-92370). {KD}

产品信息

PA5-92370

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

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免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_0017711)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.48 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2806456

产品详细信息

Immunogen sequence: AGYVFRDKVM SEFNNNFRQQ MENYPKNNHT ASILDRMQAD FKCCGAANYT DWEKIPSMSK NRVPDSCCIN VTVGCGINFN EKAIHKEGCV EKIGGWLRKN V; Positive Samples: A375, 22Rv1, Mouse kidney, Mouse spleen; Cellular Location: Cell membrane, Endosome, Late endosome membrane, Lysosome membrane, Melanosome, Multi-pass membrane protein, multivesicular body

靶标信息

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30

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基因别名: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30

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UniProt ID: (Human) P08962

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Entrez Gene ID: (Human) 967

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功能

protein binding protein complex binding

参与通路