Invitrogen

CD8a Monoclonal Antibody (OKT8 (OKT-8)), PE, eBioscience™

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CD8a Antibody (12-0086-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and Mouse IgG2a kappa Isotype Control, PE (Product # 12-4724-82) (left) or CD8a Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-0086-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.03 µg)/test

查看29篇已发表文章 29篇已发表文章

产品规格

种属反应

Human

已发表种属

Bovine, Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

OKT8 (OKT-8)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10732344

产品详细信息

Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Product # 14-0088 and clone HIT8a, Product # 14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins.

Cancer communications (London, England)

Qiao Y,Chen J,Wang X,Yan S,Tan J,Xia B,Chen Y,Lin K,Zou F,Liu B,He X,Zhang Y,Zhang X,Zhang H,Wu X,Lu L

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2023

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2023

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

12-0086-42 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 UTC 2023

Single-cell profiling of the antigen-specific response to BNT162b2 SARS-CoV-2 RNA vaccine.

Nature communications

Kramer KJ,Wilfong EM,Voss K,Barone SM,Shiakolas AR,Raju N,Roe CE,Suryadevara N,Walker LM,Wall SC,Paulo A,Schaefer S,Dahunsi D,Westlake CS,Crowe JE,Carnahan RH,Rathmell JC,Bonami RH,Georgiev IS,Irish JM

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Thu Jun 16 00:00:00 UTC 2022

Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediated homology-directed repair.

STAR protocols

Moosmann C,Müller TR,Busch DH,Schober K

12-0086-42 was used in Flow Cytometry to demonstrate the use of CRISPR-Cas9 for simultaneous editing of T-cell receptor α- and β-chains in primary human T cells.

Fri Mar 18 00:00:00 UTC 2022

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

12-0086-42 was used in Flow cytometry/Cell sorting to explore the expression of iNKT cells in psoriasis patients and the effect of secukinumab on them.

Wed Feb 23 00:00:00 UTC 2022

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

12-0086-42 was used in Flow cytometry/Cell sorting to explore the expression of iNKT cells in psoriasis patients and the effect of secukinumab on them.

Wed Feb 23 00:00:00 UTC 2022

Longitudinal single-cell profiling reveals molecular heterogeneity and tumor-immune evolution in refractory mantle cell lymphoma.

Nature communications

Zhang S,Jiang VC,Han G,Hao D,Lian J,Liu Y,Zhang R,McIntosh J,Wang R,Dang M,Dai E,Wang Y,Santos D,Badillo M,Leeming A,Chen Z,Hartig K,Bigcal J,Zhou J,Kanagal-Shamanna R,Ok CY,Lee H,Steiner RE,Zhang J,Song X,Nair R,Ahmed S,Rodriquez A,Thirumurthi S,Jain P,Wagner-Bartak N,Hill H,Nomie K,Flowers C,Futreal A,Wang L,Wang M

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon May 17 00:00:00 UTC 2021

Single-cell transcriptome analysis of the heterogeneous effects of differential expression of tumor PD-L1 on responding TCR-T cells.

Theranostics

Ding R,Liu S,Wang S,Chen H,Wang F,Xu Q,Zhu L,Dong X,Gu Y,Zhang X,Chao CC,Gao Q

12-0086-42 was used in Flow Cytometry to evaluate how tumor expression of PD-L1 affects T cell functionality in anti-cancer TCR-T cell therapy.

Mon Aug 02 00:00:00 UTC 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

12-0086-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 UTC 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

12-0086-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 UTC 2021

New Bicistronic TALENs Greatly Improve Genome Editing.

Current protocols in stem cell biology

Martín-Fernández JM,Fleischer A,Vallejo-Diez S,Palomino E,Sánchez-Gilabert A,Ruiz R,Bejarano Y,Llinàs P,Gayá A,Bachiller D

12-0086-42 was used in Flow Cytometry /test) to present data regarding the use of a new, more efficient type of transcription activator-like effector nuclease (TALEN) for gene editing.

Sun Mar 01 00:00:00 UTC 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Fri Mar 01 00:00:00 UTC 2019

Prognostic Values of CD38⁺CD101⁺PD1⁺CD8⁺ T Cells in Pancreatic Cancer.

Immunological investigations

Zhang M,Yang J,Zhou J,Gao W,Zhang Y,Lin Y,Wang H,Ruan Z,Ni B

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon Jul 01 00:00:00 UTC 2019

Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5.

BMC biotechnology

Lv Y,Xiao FJ,Wang Y,Zou XH,Wang H,Wang HY,Wang LS,Lu ZZ

12-0086-42 was used in Flow Cytometry to show the HAdV-11p fiber pseudotyped HAdV-5 could effectively transduce human T cells when human EF1a promoter was used to control the expression of transgene, suggesting its possible application in T cell immunocellular therapy.

Wed Apr 24 00:00:00 UTC 2019

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

Extensive T cell cross-reactivity between diverse seasonal influenza strains in the ferret model.

Scientific reports

Reber AJ,Music N,Kim JH,Gansebom S,Chen J,York I

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Tue Apr 17 00:00:00 UTC 2018

Disruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma.

Frontiers in pharmacology

Guo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Thu Sep 28 00:00:00 UTC 2023

Essential role for CCR6 in certain inflammatory diseases demonstrated using specific antagonist and knockin mice.

JCI insight

Robert R,Ang C,Sun G,Juglair L,Lim EX,Mason LJ,Payne NL,Bernard CC,Mackay CR

12-0086 was used in Flow cytometry/Cell sorting to investigate the role of CCR6 in the recruitment of T cells to inflammatory sites.

Thu Aug 03 00:00:00 UTC 2017

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

12-0086-42 was used in Flow Cytometry to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 UTC 2016

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2016

Increased circulating IL-9-producing CD8⁺ T cells are associated with eosinophilia and high FeNO in allergic asthmatics.

Experimental and therapeutic medicine

Wang W,Cheng ZS,Chen YF,Lin YH

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2016

Large-Scale Purification of r28M: A Bispecific scFv Antibody Targeting Human Melanoma Produced in Transgenic Cattle.

PloS one

Spiesberger K,Paulfranz F,Egger A,Reiser J,Vogl C,Rudolf-Scholik J,Mayrhofer C,Grosse-Hovest L,Brem G

12-0086-42 was used in Flow cytometry/Cell sorting to study how the bispecific scFv antibody r28M produced in transgenic cattle targets human melanoma

Mon Jun 27 00:00:00 UTC 2016

TL1A increases expression of CD25, LFA-1, CD134 and CD154, and induces IL-22 and GM-CSF production from effector CD4 T-cells.

PloS one

Reichwald K,Jørgensen TZ,Skov S

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Tue Nov 10 00:00:00 UTC 2015

Autologous Graft versus Host Disease: An Emerging Complication in Patients with Multiple Myeloma.

Bone marrow research

Batra A,Cottler-Fox M,Harville T,Rhodes-Clark BS,Makhoul I,Nakagawa M

12-0086 was used in Flow cytometry/Cell sorting to compare expression of cell surface molecules on hematopoietic progenitor cell products between healthy individuals and sufferers of autologous graft versus host disease.

Fri May 30 00:00:00 UTC 2014

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon Nov 11 00:00:00 UTC 2013

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Fri Oct 13 00:00:00 UTC 2017

Epigenetic biomarkers of T-cells in human glioma.

Epigenetics

Wiencke JK,Accomando WP,Zheng S,Patoka J,Dou X,Phillips JJ,Hsuang G,Christensen BC,Houseman EA,Koestler DC,Bracci P,Wiemels JL,Wrensch M,Nelson HH,Kelsey KT

12-0086 was used in Flow cytometry/Cell sorting to develop a new assay to capture glioma immune biology based on quantitative methylation specific PCR (qMSP) of two T-cell genes.

Sat Dec 01 00:00:00 UTC 2012

TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.

Molecular immunology

Arimura Y,Vang T,Tautz L,Williams S,Mustelin T

12-0086 was used in Flow cytometry/Cell sorting to investigate how PTP-PEST is down-regulated following T cell activation, and how that can lead to reduced IL-2 secretion following TCR and CD28 stimulation.

Sun Jun 01 00:00:00 UTC 2008

Discordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine.

Vaccine

Co MD,Orphin L,Cruz J,Pazoles P,Rothman AL,Ennis FA,Terajima M

Published figure using CD8a monoclonal antibody (Product # 12-0086-42) in Flow Cytometry

Mon Apr 07 00:00:00 UTC 2008

生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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基因别名: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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功能

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

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