Invitrogen

CD98 Monoclonal Antibody (RL388), PE, eBioscience™

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CD98 Antibody (12-0981-81) in Flow

Staining of mouse splenocytes with Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (left, open histogram) or Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (right, open histogram) and Anti-Mouse CD98 FITC (left, filled histogram) and 0.25 µg of Anti-Mouse CD98 PE (right, filled histogram). Total viable cells were used for analysis.

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产品信息

12-0981-81

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

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流式细胞分析 (Flow)

0.5 µg/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

RL388

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465792

产品详细信息

Description: The RL388 monoclonal antibody reacts with mouse CD98, also known as 4F2 antigen and Ly-10. RL388 detects a disulfide-linked heterodimer complex (130 kDa) composed of a glycosylated heavy (86 kDa) and a nonglycosylated light (39 kDa) subunits. CD98 is expressed on the surface of activated lymphocytes and is involved in cell activation and calcium flux events.

Applications Reported: The RL388 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RL388 antibody has been tested by flow cytometric analysis of activated and resting mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD98 (4F2) is a type II transmembrane glycoprotein which serves as the heavy chain of the heterodimeric amino acid transporters (HATs). CD98, linked to various light chains by disulfide bond, is responsible for cell surface expression and basolateral localization of this transporter complex in polarized epithelial cells and also interacts with beta1 integrins and increases their affinity for ligand. Besides its roles in amino acid transport, CD98 is thus involved in cell fusion and activation. It is implicated in regulation of cellular differentiation, growth and apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Glabridin, a bioactive component of licorice, ameliorates diabetic nephropathy by regulating ferroptosis and the VEGF/Akt/ERK pathways.

Molecular medicine (Cambridge, Mass.)

Tan H,Chen J,Li Y,Li Y,Zhong Y,Li G,Liu L,Li Y

12-0981-81 was used in Western Blot to reveale protective effects of Glab on the kidney of diabetic rats, which might exert by suppressing ferroptosis and the VEGF/Akt/ERK pathway.

Fri May 20 00:00:00 UTC 2022

Metabolic requirements of NK cells during the acute response against retroviral infection.

Nature communications

Littwitz-Salomon E,Moreira D,Frost JN,Choi C,Liou KT,Ahern DK,O'Shaughnessy S,Wagner B,Biron CA,Drakesmith H,Dittmer U,Finlay DK

12-0981-81 was used in Flow cytometry/Cell sorting to show that following infection natural killer cells increase nutrient uptake, including amino acids and iron, and reprogram their metabolic machinery by increasing glycolysis and mitochondrial metabolism.

Fri Sep 10 00:00:00 UTC 2021

Noc4L-Mediated Ribosome Biogenesis Controls Activation of Regulatory and Conventional T Cells.

Cell reports

Zhu X,Zhang W,Guo J,Zhang X,Li L,Wang T,Yan J,Zhang F,Hou B,Gao N,Gao GF,Zhou X

12-0981 was used in Flow cytometry/Cell sorting to identify the control of Treg activation and immune tolerance maintenance is due to Noc4L mediated ribosome biogenesis.

Tue Apr 23 00:00:00 UTC 2019

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

12-0981 was used in Flow cytometry/Cell sorting to elucidate the crosstalk between Raptor (mTORC1) and Rictor (mTORC2) to variegate cytokine signalling in controlling natural killer cell maturation and effector function.

Mon Nov 19 00:00:00 UTC 2018

The Kinase mTORC1 Promotes the Generation and Suppressive Function of Follicular Regulatory T Cells.

Immunity

Xu L,Huang Q,Wang H,Hao Y,Bai Q,Hu J,Li Y,Wang P,Chen X,He R,Li B,Yang X,Zhao T,Zhang Y,Wang Y,Ou J,Liang H,Wu Y,Zhou X,Ye L

12-0981-81 was used in Flow Cytometry to study the effect of mTORC1 on Tfr cell differentiation by activating the TCF-1-Bcl-6 axis during immunization or infection.

Tue Sep 19 00:00:00 UTC 2017

CD98 regulates vascular smooth muscle cell proliferation in atherosclerosis.

Atherosclerosis

Baumer Y,McCurdy S,Alcala M,Mehta N,Lee BH,Ginsberg MH,Boisvert WA

12098181 was used in immunohistochemistry to ask if CD98 heavy chain expressed on vascular smooth muscle cells plays a role in shaping the morphology of atherosclerotic plaques by regulating vascular smooth muscle cell function

Sun Jan 01 00:00:00 UTC 2017

Metabolic maintenance of cell asymmetry following division in activated T lymphocytes.

Nature

Verbist KC,Guy CS,Milasta S,Liedmann S,Kamiński MM,Wang R,Green DR

12-0981 was used in Flow cytometry/Cell sorting to investigate the mechanisms that sustain differential cell fates in daughter T cells after activation by an antigen-presenting cell.

Thu Apr 21 00:00:00 UTC 2016

Immunomodulatory effect of captopril and local irradiation on myeloid-derived suppressor cells.

Radiation oncology journal

Cho WK,Shin SW,Kim SY,Hong CW,Choi C,Park W,Noh JM

12-0981 was used in Flow cytometry/Cell sorting to explore the anti-tumour effects of the enzyme inhibitor, Captopril.

Thu Sep 01 00:00:00 UTC 2016

Angiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype.

Oncoimmunology

Shrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CW

12-0981 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype.

Tue Jan 12 00:00:00 UTC 2021

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals.

Nature immunology

Salmond RJ,Brownlie RJ,Morrison VL,Zamoyska R

12-0981 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity.

Mon Sep 01 00:00:00 UTC 2014

mTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function.

Journal of immunology (Baltimore, Md. : 1950)

Donnelly RP,Loftus RM,Keating SE,Liou KT,Biron CA,Gardiner CM,Finlay DK

12-0981 was used in Flow cytometry/Cell sorting to investigate whether mTORC1-controlled metabolism underpins normal natural killer cell proinflammatory functions.

Sat Nov 01 00:00:00 UTC 2014

Dependence of proliferative vascular smooth muscle cells on CD98hc (4F2hc, SLC3A2).

The Journal of experimental medicine

Fogelstrand P,Féral CC,Zargham R,Ginsberg MH

12-0981 was used in Flow cytometry/Cell sorting to establish the importance of CD98hc in vascular smooth muscle cell function and survival.

Mon Oct 26 00:00:00 UTC 2009

生物信息学

蛋白别名: 4F2 cell-surface antigen heavy chain; 4F2hc; Amino acid transporter heavy chain SLC3A2; antigen identified by monoclonal antibodies 4F2; CD98; CD98 antigen; CD98 heavy chain; Solute carrier family 3 member 2; type II transmembrane protein

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基因别名: 4F2; 4F2HC; AI314110; Cd98; Ly-10; Ly-m10; Ly10; Mdu1; Mgp-2hc; NACAE; Slc3a2

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UniProt ID: (Mouse) P10852

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Entrez Gene ID: (Mouse) 17254

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功能

double-stranded RNA binding catalytic activity protein binding neutral amino acid transmembrane transporter activity cation binding poly(A) RNA binding

参与通路

amino acid transmembrane transport carbohydrate metabolic process transport amino acid transport neutral amino acid transport response to exogenous dsRNA leucine import