Invitrogen

CDK1 Monoclonal Antibody (A17)

CDK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CDK1 Antibody (33-1800) in IHC (P)

Immunohistochemistry analysis of CDC2/CDK1 showing staining in the cytoplasm and membrane of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15... View More

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CDK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HeLa cells were transfected with CDK1 siRNA and decrease in signal intensity was observed in western blot application (Fig a) using Anti-CDK1 Mouse Monoclonal Antibody (Product # 33-1800). Densitometric analysis of this western blot is shown in histogram (Fig b). {KD}

产品信息

33-1800

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:50

免疫组化（冰冻） (IHC (F))

5-10 µg/mL

免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

0.1-1.0 µg/mL

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免疫沉淀 (IP)

5 µg

其他PubMed (Misc)

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产品规格

种属反应

Amphibian, Chicken, Human, Mouse, Rat, Xenopus

已发表种属

Human, Mouse, Xenopus

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

A17

抗原

Recombinant protein consisiting of the carboxy-terminal two-thirds of the Xenopus p34cdc2

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533105

靶标信息

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inhibiting WEE1 Augments the Antitumor Efficacy of Cisplatin in Urothelial Carcinoma by Enhancing the DNA Damage Process.

Cells

Su YL,Xiao LY,Huang SY,Wu CC,Chang LC,Chen YH,Luo HL,Huang CC,Liu TT,Peng JM

33-1800 was used in Western Blot to investigate the antitumor efficacy of the Wee1 inhibitor (AZD-1775) alone or in combination with cisplatin was evaluated in urothelial carcinoma (UC) cell lines and a xenograft mouse model.

Thu May 25 00:00:00 UTC 2023

CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition.

Nature

Gallo D,Young JTF,Fourtounis J,Martino G,Álvarez-Quilón A,Bernier C,Duffy NM,Papp R,Roulston A,Stocco R,Szychowski J,Veloso A,Alam H,Baruah PS,Fortin AB,Bowlan J,Chaudhary N,Desjardins J,Dietrich E,Fournier S,Fugère-Desjardins C,Goullet de Rugy T,Leclaire ME,Liu B,Bhaskaran V,Mamane Y,Melo H,Nicolas O,Singhania A,Szilard RK,Tkáč J,Yin SY,Morris SJ,Zinda M,Marshall CG,Durocher D

Published figure using CDK1 monoclonal antibody (Product # 33-1800) in Western Blot

Fri Apr 01 00:00:00 UTC 2022

Pancreatic tumor eradication via selective Pin1 inhibition in cancer-associated fibroblasts and T lymphocytes engagement.

Nature communications

Liu J,Wang Y,Mu C,Li M,Li K,Li S,Wu W,Du L,Zhang X,Li C,Peng W,Shen J,Liu Y,Yang D,Zhang K,Ning Q,Fu X,Zeng Y,Ni Y,Zhou Z,Liu Y,Hu Y,Zheng X,Wen T,Li Z,Liu Y

33-1800 was used in Western Blot experiments to formulate a DNA-barcoded micellular system (DMS) encapsulating the Pin1 inhibitor AG17724.

Mon Jul 25 00:00:00 UTC 2022

Discovery of an Orally Bioavailable and Selective PKMYT1 Inhibitor, RP-6306.

Journal of medicinal chemistry

Szychowski J,Papp R,Dietrich E,Liu B,Vallée F,Leclaire ME,Fourtounis J,Martino G,Perryman AL,Pau V,Yin SY,Mader P,Roulston A,Truchon JF,Marshall CG,Diallo M,Duffy NM,Stocco R,Godbout C,Bonneau-Fortin A,Kryczka R,Bhaskaran V,Mao D,Orlicky S,Beaulieu P,Turcotte P,Kurinov I,Sicheri F,Mamane Y,Gallant M,Black WC

33-1800 was used in ELISA to investigate the pharmacological role of PKMYT1.

Thu Aug 11 00:00:00 UTC 2022

Downregulation of Stem-Loop Binding Protein by Nicotine via α7-Nicotinic Acetylcholine Receptor and Its Role in Nicotine-Induced Cell Transformation.

Toxicological sciences : an official journal of the Society of Toxicology

Sun Q,Chen D,Raja A,Grunig G,Zelikoff J,Jin C

33-1800 was used in Western Blotting to propose that the SLBP depletion and polyadenylation of canonical histone mRNAs via activation of α7-nAChR and a series of downstream signal transduction pathways are critical for nicotine-induced cell transformation and potential carcinogenesis.

Sat Sep 24 00:00:00 UTC 2022

Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway.

Aging

Jeon S,Yoon YS,Kim HK,Han J,Lee KM,Seol JE,Cho SK,Park CS

33-1800 was used in Western Blotting to demonstrate reversion of the decreased protein level of collagen I after the reintroduction of the CRBN gene back into CRBN KO MEFs.

Wed Mar 03 00:00:00 UTC 2021

Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway.

Aging

Jeon S,Yoon YS,Kim HK,Han J,Lee KM,Seol JE,Cho SK,Park CS

33-1800 was used in Western Blotting to demonstrate reversion of the decreased protein level of collagen I after the reintroduction of the CRBN gene back into CRBN KO MEFs.

Wed Mar 03 00:00:00 UTC 2021

Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy.

Scientific reports

Moretti FA,Giardino G,Attenborough TCH,Gkazi AS,Margetts BK,la Marca G,Fairbanks L,Crompton T,Gaspar HB

Published figure using CDK1 monoclonal antibody (Product # 33-1800) in Western Blot

Wed Dec 01 00:00:00 UTC 2021

PADI3 plays an antitumor role via the Hsp90/CKS1 pathway in colon cancer.

Cancer cell international

Chai Z,Wang L,Zheng Y,Liang N,Wang X,Zheng Y,Zhang Z,Zhao C,Zhu T,Liu C

Published figure using CDK1 monoclonal antibody (Product # 33-1800) in Western Blot

Sat Nov 04 00:00:00 UTC 2023

Paxillin and embryonic PolyAdenylation Binding Protein (ePABP) engage to regulate androgen-dependent Xenopus laevis oocyte maturation - A model of kinase-dependent regulation of protein expression.

Molecular and cellular endocrinology

Miedlich SU,Taya M,Young MR,Hammes SR

33-1800 was used in Western Blotting to study whether paxillin enhances Mos translation by interacting with embryonic PolyAdenylation Binding Protein on polyadenylated Mos mRNA.

Thu Jun 15 00:00:00 UTC 2017

Enhanced expression of mitochondrial superoxide dismutase leads to prolonged in vivo cell cycle progression and up-regulation of mitochondrial thioredoxin.

Free radical biology & medicine

Kim A,Joseph S,Khan A,Epstein CJ,Sobel R,Huang TT

33-1800 was used in immunohistochemistry - paraffin section to investigate the role of Mn superoxide dismutase in tissue regeneration using transgenic mice

Tue Jun 01 00:00:00 UTC 2010

Abnormal expression of differentiation related proteins and proteoglycan core proteins in the urothelium of patients with interstitial cystitis.

The Journal of urology

Hauser PJ,Dozmorov MG,Bane BL,Slobodov G,Culkin DJ,Hurst RE

33-1800 was used in Immunohistochemistry to determine the origins of the loss of the glycosaminoglycan layer and investigate more fully the altered differentiation of the urothelium in interstitial cystitis.

Fri Feb 01 00:00:00 UTC 2008

CDC2/CDK1 expression in esophageal adenocarcinoma and precursor lesions serves as a diagnostic and cancer progression marker and potential novel drug target.

The American journal of surgical pathology

Hansel DE,Dhara S,Huang RC,Ashfaq R,Deasel M,Shimada Y,Bernstein HS,Harmon J,Brock M,Forastiere A,Washington MK,Maitra A,Montgomery E

Tue Mar 01 00:00:00 UTC 2005

Alpha(v)beta3 integrin expression up-regulates cdc2, which modulates cell migration.

The Journal of cell biology

Manes T,Zheng DQ,Tognin S,Woodard AS,Marchisio PC,Languino LR

Mon May 26 00:00:00 UTC 2003

生物信息学

蛋白别名: ABL1; CDK1; CDK1-A; cell cycle controller CDC2; cell cycle p34 CDC2 kinase protein; Cell division control protein 2 homolog; Cell division control protein 2 homolog 1; Cell division control protein 2-A; cell division cycle 2 homolog A; cell division cycle 2 like; cell division cycle 2, G1 to S and G2 to M; Cell division cycle control protein 2; cell division cycle control protein 2a; Cell division protein kinase 1; Cell division protein kinase 1-A; Cyclin-dependent kinase 1; Cyclin-dependent kinase 1-A; DKFZp686L20222; KCIP-1; maturation-promoting factor; MGC111195; MGC53829; OTTHUMP00000019660; P34 CDK1; P34 MGC111195; p34 protein kinase; p34 protein kinase 1; Protein 1054; unnamed protein product

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基因别名: cdc-2; CDC2; cdc2-a; CDC28A; Cdc2a; cdc2x1.1; CDK1; cdk1-a; cdk1-b; CDKN1; MPF; p34; P34CDC2; PSTAIR; xcdc2; XELAEV_18036802mg