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CREB Antibody (PA1-850) in ICC/IF
Immunofluorescent analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB polyclonal antibody (Product # PA1-850) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated... View More
产品信息
PA1-850
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues K(123) R R E I L S R R P S Y R K(136) of human CREB.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2086016
产品详细信息
PA1-850 detects both the phosphorylated and non-phosphorylated forms of cyclic-AMP response element binding protein (CREB) from rat, mouse, and human samples.
PA1-850 has been successfully used in ChIP, Western blot, ICC/IF and IHC-P procedures. By Western blot, this antibody detects an ~43 kDa protein representing CREB from GH4 cell extract.
The PA1-850 immunogen is a synthetic peptide corresponding to residues K(123) R R E I L S R R P S Y R K(136) of human CREB. This sequence is completely conserved between human, bovine, canine, mouse, pig, sheep, and rat CREB protein. The PA1-850 immunizing peptide (Cat. # PEP-142) is available for use in neutralization and control experiments.
靶标信息
CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: active transcription factor CREB; cAMP response element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein
基因别名: 2310001E10Rik; 3526402H21Rik; AV083133; CREB; CREB-1; CREB1
UniProt ID: (Human) P16220, (Mouse) Q01147, (Rat) P15337
Entrez Gene ID: (Human) 1385, (Mouse) 12912, (Rat) 81646
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
transcriptional activator activity, RNA polymerase II transcription factor binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription cofactor activity
protein binding
enzyme binding
Hsp70 protein binding
histone acetyltransferase binding
cAMP response element binding
identical protein binding
arrestin family protein binding
DNA binding
double-stranded DNA binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein phosphorylation
transforming growth factor beta receptor signaling pathway
axonogenesis
lactation
memory
circadian rhythm
regulation of cell size
response to organic substance
negative regulation of transcription by competitive promoter binding
pituitary gland development
cell differentiation
mammary gland development
positive regulation of transforming growth factor beta3 production
secretory granule organization
response to glucagon
positive regulation of multicellular organism growth
response to drug
regulation of circadian rhythm
regulation of apoptotic process
positive regulation of fat cell differentiation
positive regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone secretion
positive regulation of lipid biosynthetic process
rhythmic process
protein stabilization
lung epithelium development
lung saccule development
Type I pneumocyte differentiation
cellular response to zinc ion
cellular response to growth factor stimulus
response to hypoxia
signal transduction
axon guidance
aging
visual learning
response to activity
viral process
chemotaxis to arachidonic acid
response to nicotine
cellular response to hepatocyte growth factor stimulus
cellular response to platelet-derived growth factor stimulus
response to antidepressant
positive regulation of apoptotic process
regulation of fibroblast proliferation
regulation of glial cell proliferation
cellular response to fatty acid
positive regulation of long-term synaptic potentiation
negative regulation of neuron death
response to L-glutamate
cellular response to nerve growth factor stimulus
cellular response to insulin-like growth factor stimulus
