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Cas9 Antibody (MA5-23519) in ICC/IF
Immunofluorescent detection of Cas9 in Hela cells transiently transfected with a Flag-tagged Cas9 expression vector using a Cas9 monoclonal antibody (Product # MA5-23519). 48 hours post transfection, the cells were fixed in 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the Cas9 (fig 4A and B) or with an anti-Flag (fig 4C) antibody at 4°C overnight, followed by incubation with an anti-mouse secondary antibody coupled to AlexaFluor 488 for 1 hr at RT.... View More
产品信息
MA5-23519
应用
建议稀释比
已发表文章
产品规格
种属反应
Bacteria
已发表种属
Bacteria,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
7A9
抗原
Cas9 nuclease (CRISPR-associated protein 9).
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.05% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610639
产品详细信息
MA5-23519 is derived from Streptococcus pyogenes.
靶标信息
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. The system is unique and flexible due to its dependence on RNA as the element that targets the nuclease to a desired DNA sequence. It can be used to induce indel mutations, specific sequence replacements/insertions, and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9). SaCas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cas9; CRISPR-Cas9; CRISPR9; s aureus
