Invitrogen

CXCL10 Polyclonal Antibody, PeproTech®

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CXCL10 Antibody (500-P93-1MG) in WB

Western Blot: To detect hIP-10 by Western Blot analysis CXCL10 Polyclonal Antibody (Product # 500-P93-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P93-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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中和实验 (Neu)

- -

体外检测 (IV)

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免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human IP-10 (CXCL10)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930864

产品详细信息

AA Sequence of recombinant protein: VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IP-10 (CXCL10). Anti-Human IP-10 (CXCL10)-specific antibody was purified by affinity chromatography employing an immobilized Human IP-10 (CXCL10) matrix.

Sandwich ELISA: To detect Human IP-10 (CXCL10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IP-10 (CXCL10) (500-P93Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IP-10 (CXCL10).

Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Urinary CXCL10 Chemokine Is Associated With Alloimmune and Virus Compartment-Specific Renal Allograft Inflammation.

Transplantation

Ho J,Schaub S,Wiebe C,Gao A,Wehmeier C,Koller MT,Hirsch HH,Hopfer H,Nickerson P,Hirt-Minkowski P

500-P93 was used in Enzyme-linked immunosorbent assay to conclude that urinary CXCL10 reflects subclinical inflammation within the tubulointerstitial and peritubular capillary spaces, but not the vascular/systemic compartments.

Thu Mar 01 00:00:00 UTC 2018

Lethal Nipah virus infection induces rapid overexpression of CXCL10.

PloS one

Mathieu C,Guillaume V,Sabine A,Ong KC,Wong KT,Legras-Lachuer C,Horvat B

Mon Jul 23 00:00:00 UTC 2012

Tumor-promoting circuits that regulate a cancer-related chemokine cluster: dominance of inflammatory mediators over oncogenic alterations.

Cancers

Leibovich-Rivkin T,Buganim Y,Solomon H,Meshel T,Rotter V,Ben-Baruch A

500-P93 was used in Enzyme-linked immunosorbent assay to use an unbiased model system to propose that inflammatory mediators of the tumor milieu have dominating roles over oncogenic modifications in dictating the expression of a pro-malignancy chemokine readout.

Fri Jan 20 00:00:00 UTC 2012

Dysregulated expression of MIG/CXCL9, IP-10/CXCL10 and CXCL16 and their receptors in systemic sclerosis.

Arthritis research & therapy

Rabquer BJ,Tsou PS,Hou Y,Thirunavukkarasu E,Haines GK,Impens AJ,Phillips K,Kahaleh B,Seibold JR,Koch AE

Tue Feb 08 00:00:00 UTC 2011

Predominant expression of CCL2 at the tumor site of prostate cancer patients directs a selective loss of immunological tolerance to CCL2 that could be amplified in a beneficial manner.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Weinberg U,Shaked Y,Alami J,Dumont D,Friedman B,Stein A,Karin N

Fri Jan 15 00:00:00 UTC 2010

Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.

Blood

Ma Y,Visser L,Roelofsen H,de Vries M,Diepstra A,van Imhoff G,van der Wal T,Luinge M,Alvarez-Llamas G,Vos H,Poppema S,Vonk R,van den Berg A

Fri Feb 15 00:00:00 UTC 2008

Differences in mesenchymal stem cell cytokine profiles between MS patients and healthy donors: implication for assessment of disease activity and treatment.

Journal of neuroimmunology

Mazzanti B,Aldinucci A,Biagioli T,Barilaro A,Urbani S,Dal Pozzo S,Amato MP,Siracusa G,Crescioli C,Manuelli C,Bosi A,Saccardi R,Massacesi L,Ballerini C

Wed Aug 13 00:00:00 UTC 2008

CXCL10-CXCR3 interactions play an important role in the pathogenesis of acute graft-versus-host disease in the skin following allogeneic stem-cell transplantation.

Blood

Piper KP,Horlock C,Curnow SJ,Arrazi J,Nicholls S,Mahendra P,Craddock C,Moss PA

Sat Dec 01 00:00:00 UTC 2007

Elevated serum interferon-gamma-inducible chemokine-10/CXC chemokine ligand-10 in autoimmune primary adrenal insufficiency and in vitro expression in human adrenal cells primary cultures after stimulation with proinflammatory cytokines.

The Journal of clinical endocrinology and metabolism

Rotondi M,Falorni A,De Bellis A,Laureti S,Ferruzzi P,Romagnani P,Buonamano A,Lazzeri E,Crescioli C,Mannelli M,Santeusanio F,Bellastella A,Serio M

Fri Apr 01 00:00:00 UTC 2005

Chronic gene delivery of interferon-inducible protein 10 through replication-competent retrovirus vectors suppresses tumor growth.

Cancer gene therapy

Sun Y,Finger C,Alvarez-Vallina L,Cichutek K,Buchholz CJ

Tue Nov 01 00:00:00 UTC 2005

CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.

The European respiratory journal

Pilette C,Francis JN,Till SJ,Durham SR

Tue Jun 01 00:00:00 UTC 2004

CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.

The European respiratory journal

Pilette C,Francis JN,Till SJ,Durham SR

Tue Jun 01 00:00:00 UTC 2004

Elevated serum levels of the CXCR3 chemokine ITAC are associated with the development of transplant coronary artery disease.

Circulation

Kao J,Kobashigawa J,Fishbein MC,MacLellan WR,Burdick MD,Belperio JA,Strieter RM

Tue Apr 22 00:00:00 UTC 2003

Interleukin 12-activated lymphocytes influence tumor genetic programs.

Cancer research

Cavallo F,Quaglino E,Cifaldi L,Di Carlo E,André A,Bernabei P,Musiani P,Forni G,Calogero RA

Sun Apr 15 00:00:00 UTC 2001

Differential chemokine expression in tissues involved by Hodgkin's disease: direct correlation of eotaxin expression and tissue eosinophilia.

Blood

Teruya-Feldstein J,Jaffe ES,Burd PR,Kingma DW,Setsuda JE,Tosato G

Thu Apr 15 00:00:00 UTC 1999

生物信息学

蛋白别名: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; CXC; CXC motif chemokine; CXCL; gamma IP10; Gamma-IP10; H-IP-10; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10

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基因别名: C7; crg-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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UniProt ID: (Human) P02778

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Entrez Gene ID: (Human) 3627

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功能

receptor binding protein binding chemokine activity heparin binding cAMP-dependent protein kinase regulator activity CXCR3 chemokine receptor binding chemokine

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