Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
CYP46A1 Antibody (PA5-121211) in WB
Western blot analysis of CYP46A1 in extracts of various cell lines. Samples were incubated with CYP46A1 Polyclonal antibody (Product # PA5-121211) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-121211
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of human CYP46A1 (NP_006659.1).
偶联物
形式
Liquid
浓度
0.9 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914783
产品详细信息
Positive test controls include: U-87MG, U-251MG, MCF7, Mouse liver, Mouse brain, Rat liver. The target is usually found in the following locations: Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein.
Immunogen sequence: HRARSRYEHI PGPPRPSFLL GHLPCFWKKD EVGGRVLQDV FLDWAKKYGP VVRVNVFHKT SVIVTSPESV KKFLMSTKYN KDSKMYRALQ TVFGERLFGQ GLVSECNYER WHKQRRVIDL AFSRSSLVSL METFNEKAEQ LVEILEAKAD GQTPVSMQDM LTYTAMDILA KAAFGMETSM LLGAQKPLSQ AVKLMLEGIT ASRNTLAKFL PGKRKQLREV RESIRFLRQV GRDWVQRRRE ALKRGEEVPA DILTQIL
靶标信息
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: CH24H; Cholesterol 24-hydroxylase; Cholesterol 24-monooxygenase; Cholesterol 24S-hydroxylase; cholestrol 24-hydroxylase; Cytochrome P450 46A1; cytochrome P450, 46 (cholesterol 24-hydroxylase); cytochrome P450, 46a1 (cholesterol 24-hydroxylase); cytochrome P450, family 46, subfamily A, polypeptide 1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
基因别名: CP46; CYP46; CYP46A1
UniProt ID: (Human) Q9Y6A2, (Mouse) Q9WVK8
Entrez Gene ID: (Human) 10858, (Rat) 362782, (Mouse) 13116
iron ion binding
steroid hydroxylase activity
heme binding
cholesterol 24-hydroxylase activity
monooxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
metal ion binding
oxidoreductase
oxygenase
metabolite interconversion enzyme
