Rockland

Carboxypeptidase A Polyclonal Antibody, HRP

产品信息

200-4352-0100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:3,000-1:10,000

免疫沉淀 (IP)

1:300-1:2,000

产品规格

种属反应

Bovine

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carboxypeptidase A [Bovine Pancreas]

查看免疫原

偶联物

HRP HRP HRP

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Ion-exchange chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

内含物

0.01% gentamicin sulfate

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas]. Cross reactivity against Carboxypeptidase A from other tissues and species may occur but have not been specifically determined.

靶标信息

CPA1, also known as CPA, is a 419 amino acid secreted monomeric protein that is highly expressed in pancreatic tissue. Functioning as a pancreatic exopeptidase, CPA1 uses zinc as a cofactor to catalyze the release of C-terminal amino acids from a variety of proteins, thereby playing a key role in protein digestion and degradation. Via its catalytic activity, CPA1 is also thought to be involved in zymogen (proenzyme) inhibition, probably functioning to block enzyme activation pathways. Abnormal levels of CPA1 are associated with pancreatic cancer, suggesting a possible role in either tumor progression or tumor suppression events.

仅用于科研。不用于诊断过程。未经明确授权不得转售。