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Carboxypeptidase B Antibody (200-403-049-0100) in WB
Western Blot of Rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated. Lane 1: Carboxypeptidase B. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carboxypeptidase B Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 47 kDa, observed at 35 kDa for Carboxypeptidase B. Expected molecular weight for processed protein is ~34.7 kDa.
产品信息
200-403-049-0100
产品规格
种属反应
Pig
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carboxypeptidase B [Porcine Pancreas]
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl
内含物
0.01% gentamicin sulfate
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas]. Cross reactivity against Carboxypeptidase B from other tissues and species is unknown.
靶标信息
Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Carboxypeptidase B; carboxypeptidase B1 (tissue); procarboxypeptidase B
基因别名: CPB; CPB1
UniProt ID: (Pig) P09955
Entrez Gene ID: (Pig) 397341
