Rockland

Catalase Polyclonal Antibody

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Catalase Antibody (200-401-051) in WB

Western Blot of Rabbit anti-Catalase antibody. Lane 1: purified Catalase reduced. Lane 2: purified Catalase non-reduced. Load: ~1 µg per lane. Primary antibody: anti Catalase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti rabbit secondary antibody at 1:10,000 for 1.5 hours at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 59.9 kDa, 55kDa for Catalase. Other band(s): none.

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产品信息

200-401-051

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:20,000

免疫沉淀 (IP)

1:10,000-1:50,000

产品规格

种属反应

Bovine, Human, Mouse, Sheep, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-Catalase Antibody was produced by repeated immunizations with bovine liver catalase protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Ion-exchange chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Catalase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.

靶标信息

Catalase is a homotetrameric heme-containing enzyme present within the matrix of all peroxisomes. It carries out a dismutation reaction in which hydrogen peroxide is converted to water and oxygen. Human catalase has the last four amino acids (-KANL) at the extreme C-terminus for peroxisome targeting. The monomer of human catalase is 61.3 kDa in molecular size. Catalase has been implicated as an important factor in inflammation, mutagenesis, prevention of apoptosis, and stimulation of a wide spectrum of tumors. Loss of catalase leads to the human genetic disease, acatalasemia, or Takahara's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。