Invitrogen

ChAT Polyclonal Antibody

ChAT Antibody in Immunocytochemistry (ICC/IF)

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ChAT Antibody (PA5-29653) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ChAT was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: ChAT Polyclonal Antibody (Product # PA5-29653) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

ChAT Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ChAT Antibody in Immunohistochemistry (Frozen) (IHC (F))

ChAT Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-29653

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 102 and 351 of Human CHAT

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.15 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547128

产品详细信息

Recommended positive controls: IMR32, mouse brain, rat brain, DDDDK-tagged CHAT-transfected 293T.

Predicted reactivity: Mouse (93%), Rat (92%), Pig (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Focal impaired awareness seizures in a rodent model: A functional anatomy.

Epilepsia open

Adotevi N,Kapur J

PA5-29653 was used in Immunohistochemistry to highlight neuronal circuits preferentially activated during a focal awareness impaired seizure in a rodent model.

Tue Mar 01 00:00:00 UTC 2022

Focal impaired awareness seizures in a rodent model: A functional anatomy.

Epilepsia open

Adotevi N,Kapur J

PA5-29653 was used in Immunohistochemistry to highlight neuronal circuits preferentially activated during a focal awareness impaired seizure in a rodent model.

Tue Mar 01 00:00:00 UTC 2022

Pegylated Insulin-Like Growth Factor 1 attenuates Hair Cell Loss and promotes Presynaptic Maintenance of Medial Olivocochlear Cholinergic Fibers in the Cochlea of the Progressive Motor Neuropathy Mouse.

Frontiers in neurology

Bieniussa L,Kahraman B,Skornicka J,Schulte A,Voelker J,Jablonka S,Hagen R,Rak K

PA5-29653 was used in Immunohistochemistry to investigate the effect of peg-IGF-1 on the auditory system by treatment starting at postnatal day 15 (p15).

Sat Jul 16 00:00:00 UTC 2022

Targeting the tamoxifen receptor within sodium channels to block osteoarthritic pain.

Cell reports

McCollum MM,Larmore M,Ishihara S,Ng LCT,Kimura LF,Guadarrama E,Ta MC,Vien TN,Frost GB,Scheidt KA,Miller RE,DeCaen PG

PA5-29653 was used in Functional Assay to show that when evaluated using a mouse OA pain model, ND-Tam has long-lasting efficacy, which supports the potential of repurposing ND-Tam analogs as NaV antagonists for OA pain management.