Invitrogen

Collagen I Polyclonal Antibody

Collagen I Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 20

Collagen I Antibody (PA1-26204) in IHC (P)

Immunohistochemistry of Collagen I was performed in formalin fixed, paraffin embedded normal kidney using Collagen I Polyclonal Antibody (Product # PA1-26204) at a dilution of 1:100 for 4 hours at room temperature. A peroxidase goat anti-rabbit was used as a secondary antibody at 1:10,000 for 45 min at RT. Localization: Distal tubules in normal kidney tissue. Note the absence of staining of glomeruli. Stain... View More

Collagen I Antibody in Western Blot (WB)

Collagen I Antibody in Immunocytochemistry (ICC/IF)

Collagen I Antibody in Immunohistochemistry (IHC)

Collagen I Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

PA1-26204

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

查看13篇已发表文章 13篇已发表文章

免疫组化 (IHC)

查看10篇已发表文章 10篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

查看2篇已发表文章 2篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看6篇已发表文章 6篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1:5,000-1:50,000

免疫沉淀 (IP)

1:100

点杂交 (DB)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Collagen type I extracted and purified from human and bovine placenta by limited pepsin digestion and selective salt precipitation.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.15 mg/mL

纯化类型

Antigen affinity chromatography

保存液

20mM potassium phosphate, pH 8.0, with 150mM NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2260734

产品详细信息

Some class-specific anti-collagens are specific for three-dimensional epitopes, resulting in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Cross-reactivity with other human serum proteins and non-collagen extracellular matrix proteins is neglible.|Western blot: this preparation results in a native conformation of the protein. It is recommend not to add 2-ME or DTT in lysis buffer and not boiling the sample.

靶标信息

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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PA1-26204 was used in Immunohistochemistry to evaluate the possible protective potential of the prepared eco-friendly Taif rose oil nanoemulsion (ROSE-NANO) against UVB (ultraviolet B)-induced photoaging in adult male Wistar rats.

Tue Sep 19 00:00:00 UTC 2023

Episodic Binge-like Ethanol Reduces Skeletal Muscle Strength Associated with Atrophy, Fibrosis, and Inflammation in Young Rats.

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Cáceres-Ayala C,Mira RG,Acuña MJ,Brandan E,Cerpa W,Rebolledo DL

PA1-26204 was used in Immunohistochemistry-immunofluorescence to demonstrate that episodic binge-like ethanol exposure alters contractile capacity and increases fatigue by mechanisms involving atrophy, fibrosis, and inflammation, which remain for at least two weeks after ethanol clearance.

Sat Jan 14 00:00:00 UTC 2023

Phase-change composite filled natural nanotubes in hydrogel promote wound healing under photothermally triggered drug release.

Bioactive materials

Ye JJ,Li LF,Hao RN,Gong M,Wang T,Song J,Meng QH,Zhao NN,Xu FJ,Lvov Y,Zhang LQ,Xue JJ

PA1-26204 was used in ELISA, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to develop a hydrogel composite made of alginate and nanotubes with an efficient NIR-triggered release of rifampicin and outstanding antibacterial ability.

Wed Mar 01 00:00:00 UTC 2023

Engineering of extracellular matrix from human iPSC-mesenchymal progenitors to enhance osteogenic capacity of human bone marrow stromal cells independent of their age.

Frontiers in bioengineering and biotechnology

Hanetseder D,Levstek T,Teuschl-Woller AH,Frank JK,Schaedl B,Redl H,Marolt Presen D

PA1-26204 was used in Immunohistochemistry-immunofluorescence to suggest that the microenvironment comprised of iECM can enhance the osteogenic activity of BMSCs, providing a bioactive and scalable biomaterial strategy for enhancing bone regeneration in patients with delayed or failed bone healing.

Wed Aug 23 00:00:00 UTC 2023

Doxorubicin-induced modulation of TGF-β signaling cascade in mouse fibroblasts: insights into cardiotoxicity mechanisms.

Scientific reports

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PA1-26204 was used in Western Blot to reveal a dose-dependent reduction in cellular communication network factor 2 (CCN2) protein levels upon DOX treatment in both NIH3T3 and CFs, suggesting an antifibrotic activity by DOX in these fibroblasts.

Thu Nov 02 00:00:00 UTC 2023

Deletion of the mRNA stability factor ELAVL1 (HuR) in pancreatic cancer cells disrupts the tumor microenvironment integrity.

NAR cancer

McCarthy GA,Di Niro R,Finan JM,Jain A,Guo Y,Wyatt CR,Guimaraes AR,Waugh TA,Keith D,Morgan TK,Sears RC,Brody JR

PA1-26204 was used in Western Blotting to identify a novel role of tumor-intrinsic human antigen R in its ability to modify the surrounding tumor microenvironment and regulate platelet-derived growth factor AA (PDGFAA).

Thu Jun 01 00:00:00 UTC 2023

Malignant pleural mesothelioma nodules remodel their surroundings to vascularize and grow.

Translational lung cancer research

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PA1-26204 was used in Immunohistochemistry-immunofluorescence to investigate the vascularization of intrapleurally implanted human P31 and SPC111 malignant pleural mesothelioma cells.

Wed Jun 01 00:00:00 UTC 2022

The Role of PEMFs on Bone Healing: An In Vitro Study.

International journal of molecular sciences

Caliogna L,Bina V,Brancato AM,Gastaldi G,Annunziata S,Mosconi M,Grassi FA,Benazzo F,Pasta G

PA1-26204 was used in Immunocytochemistry to indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells.

Fri Nov 18 00:00:00 UTC 2022

Fucoidan-Mediated Inhibition of Fibrotic Properties in Oral Submucous Fibrosis via the MEG3/miR-181a/Egr1 Axis.

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PA1-26204 was used in Western Blotting to investigate the anti-fibrotic effect of fucoidan on oral fibrosis.

Tue Jul 05 00:00:00 UTC 2022

A new mouse model of post-traumatic joint injury allows to identify the contribution of Gli1+ mesenchymal progenitors in arthrofibrosis and acquired heterotopic endochondral ossification.

Frontiers in cell and developmental biology

Magallanes J,Liu NQ,Zhang J,Ouyang Y,Mkaratigwa T,Bian F,Van Handel B,Skorka T,Petrigliano FA,Evseenko D

PA1-26204 was used in Immunohistochemistry to demonstrate that genetic manipulation of Gli1+ cells in mice may offer a platform for identification of novel therapeutic targets to prevent knee joint dysfunction after chronic injury.

Wed Jan 25 00:00:00 UTC 2023

Deletion of fibroblast activation protein provides atheroprotection.

Cardiovascular research

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PA1-26204 was used in Western Blotting to assess the effects of constitutive Fap deletion on plaque formation in atherosclerosis-prone apolipoprotein E or low-density lipoprotein receptor knockout mice.

Sun Mar 21 00:00:00 UTC 2021

Late-life voluntary wheel running reverses age-related aortic stiffness in mice: a translational model for studying mechanisms of exercise-mediated arterial de-stiffening.

GeroScience

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PA1-26204 was used in Western Blot to investigate the role of voluntary wheel running as a murine experimental animal exercise suitable for studying the effects of exercise on human arterial stiffness.

Mon Feb 01 00:00:00 UTC 2021

Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds.

Scientific reports

Malakpour-Permlid A,Buzzi I,Hegardt C,Johansson F,Oredsson S

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunocytochemistry

Tue Mar 23 00:00:00 UTC 2021

Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds.

Scientific reports

Malakpour-Permlid A,Buzzi I,Hegardt C,Johansson F,Oredsson S

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunocytochemistry

Tue Mar 23 00:00:00 UTC 2021

Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system.

Nano convergence

Farooqi HMU,Kang B,Khalid MAU,Salih ARC,Hyun K,Park SH,Huh D,Choi KH

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunocytochemistry

Tue Feb 02 00:00:00 UTC 2021

Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system.

Nano convergence

Farooqi HMU,Kang B,Khalid MAU,Salih ARC,Hyun K,Park SH,Huh D,Choi KH

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunocytochemistry

Tue Feb 02 00:00:00 UTC 2021

Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.

Theranostics

Zhao H,Yang H,Geng C,Chen Y,Tang Y,Li Z,Pang J,Shu T,Nie Y,Liu Y,Jia K,Wang J

PA1-26204 was used in Immunohistochemistry to demonstrate that elevated IgE promotes pathological cardiac fibrosis by modulating miR-486a-5p and downstream factors, such as Smad1.

Wed Jul 28 00:00:00 UTC 2021

Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.

Theranostics

Zhao H,Yang H,Geng C,Chen Y,Tang Y,Li Z,Pang J,Shu T,Nie Y,Liu Y,Jia K,Wang J

PA1-26204 was used in Immunohistochemistry to demonstrate that elevated IgE promotes pathological cardiac fibrosis by modulating miR-486a-5p and downstream factors, such as Smad1.

Wed Jul 28 00:00:00 UTC 2021

Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.

Theranostics

Zhao H,Yang H,Geng C,Chen Y,Tang Y,Li Z,Pang J,Shu T,Nie Y,Liu Y,Jia K,Wang J

PA1-26204 was used in Immunohistochemistry to demonstrate that elevated IgE promotes pathological cardiac fibrosis by modulating miR-486a-5p and downstream factors, such as Smad1.

Wed Jul 28 00:00:00 UTC 2021

NSM00158 Specifically Disrupts the CtBP2-p300 Interaction to Reverse CtBP2-Mediated Transrepression and Prevent the Occurrence of Nonunion.

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PA1-26204 was used in Western Blotting to suggest that NSM00158 represents a new potential compound to prevent the occurrence of nonunion by disrupting CtBP2 function and impairing the assembly of the CtBP2-p300-Runx2 transcriptional complex.

Tue Jun 30 00:00:00 UTC 2020

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Molecules and cells

Chen X,Zhang W,Zhang Q,Song T,Yu Z,Li Z,Duan N,Dang X

Tue Jun 30 00:00:00 UTC 2020

Role of sialidase Neu3 and ganglioside GM3 in cardiac fibroblasts activation.

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PA1-26204 was used in Western Blot to identify pro-fibrotic pathways in primary cultures of human cardiac fibroblasts as targets for cardiac fibrosis.

Fri Sep 18 00:00:00 UTC 2020

Inhibition of Cathepsin K Alleviates Autophagy-Related Inflammation in Periodontitis-Aggravating Arthritis.

Infection and immunity

Yue Y,Yin W,Yang Q,Ren J,Tan L,Wang J,Liu J,Lu Q,Ding H,Zhan W,Hao L,Wang M

PA1-26204 was used in Immunohistochemistry (Paraffin) to investigate the immunological function of inhibiting cathepsin K in autophagy associated with peridontitis-aggravated rheumatoid arthritis.

Mon Nov 16 00:00:00 UTC 2020

Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells.

STAR protocols

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Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunohistochemistry

Fri Jun 19 00:00:00 UTC 2020

Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells.

STAR protocols

Wörsdörfer P,Rockel A,Alt Y,Kern A,Ergün S

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunohistochemistry

Fri Jun 19 00:00:00 UTC 2020

Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care.

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PA1-26204 was used in Immunocytochemistry to demonstrate that DAMA stimulates cell proliferation, cell migration, secretion of collagen type I, and the reduction of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α and suggest that the introduction of DAMA to non-healing, complex wounds helps to improve the wound milieu by providing essential structural components, cytokines, and growth factors to create an appropriate environment for wound healing.

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Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Immunohistochemistry

Sat Jun 01 00:00:00 UTC 2019

YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT.

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Lin F,Zeng Z,Song Y,Li L,Wu Z,Zhang X,Li Z,Ke X,Hu X

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Western Blot

Fri Aug 23 00:00:00 UTC 2019

Morphological and Molecular Changes in Juvenile Normal Human Fibroblasts Exposed to Simulated Microgravity.

Scientific reports

Buken C,Sahana J,Corydon TJ,Melnik D,Bauer J,Wehland M,Krüger M,Balk S,Abuagela N,Infanger M,Grimm D

PA1-26204 was used in Flow cytometry/Cell sorting to show simulated microgravity causes changes in the cytoskeleton, extracellular matrix, focal adhesion and growth behaviour of normal human dermal fibroblasts and to identify the factors involved in the assembly of the fibroblasts.

Thu Aug 15 00:00:00 UTC 2019

Simvastatin Increases the Ability of Roflumilast N-oxide to Inhibit Cigarette Smoke-Induced Epithelial to Mesenchymal Transition in Well-differentiated Human Bronchial Epithelial Cells in vitro.

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PA1-26204 was used in immunocytochemistry and western blot to discuss drugs that prevent the epithelial-mesenchymal transition.

Mon Jun 01 00:00:00 UTC 2015

Simvastatin Increases the Ability of Roflumilast N-oxide to Inhibit Cigarette Smoke-Induced Epithelial to Mesenchymal Transition in Well-differentiated Human Bronchial Epithelial Cells in vitro.

COPD

Milara J,Peiró T,Serrano A,Artigues E,Aparicio J,Tenor H,Sanz C,Cortijo J

PA1-26204 was used in immunocytochemistry and western blot to discuss drugs that prevent the epithelial-mesenchymal transition.

Mon Jun 01 00:00:00 UTC 2015

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Cardiovascular research

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PA1-26204 was used in immunohistochemistry and western blot to study the the role of increased CaSr expression in the mechanism by which calcimimetics reduce vascular smooth muscle cell mineralization

Sat Feb 01 00:00:00 UTC 2014

Roflumilast N-oxide inhibits bronchial epithelial to mesenchymal transition induced by cigarette smoke in smokers with COPD.

Pulmonary pharmacology & therapeutics

Milara J,Peiró T,Serrano A,Guijarro R,Zaragozá C,Tenor H,Cortijo J

PA1-26204 was used in Reverse phase protein array to study the epithelial to mesenchymal transition in the bronchial epithelium of patients with COPD and smokers

Fri Aug 01 00:00:00 UTC 2014

Anti-inflammatory and anti-fibrotic profile of fish oil emulsions used in parenteral nutrition-associated liver disease.

PloS one

Pastor-Clerigues A,Marti-Bonmati E,Milara J,Almudever P,Cortijo J

PA1-26204 was used in immunohistochemistry - paraffin section to investigate the effect of soybean and fish oil lipid commercial emulsions on inflammatory and profibrotic liver markers in adults with parenteral nutrition long term associate liver disease

Wed Mar 09 00:00:00 UTC 2016

Anti-inflammatory and anti-fibrotic profile of fish oil emulsions used in parenteral nutrition-associated liver disease.

PloS one

Pastor-Clerigues A,Marti-Bonmati E,Milara J,Almudever P,Cortijo J

Wed Mar 09 00:00:00 UTC 2016

miR-150 promotes renal fibrosis in lupus nephritis by downregulating SOCS1.

Journal of the American Society of Nephrology : JASN

Zhou H,Hasni SA,Perez P,Tandon M,Jang SI,Zheng C,Kopp JB,Austin H,Balow JE,Alevizos I,Illei GG

PA1-26204 was used in immunocytochemistry and western blot to study the role of SOCS1 downregulation in the mechanism by which miRNA-150 promotes renal fibrosis in patients with lupus nephritis

Sat Jun 01 00:00:00 UTC 2013

Epithelial to mesenchymal transition is increased in patients with COPD and induced by cigarette smoke.

Thorax

Milara J,Peiró T,Serrano A,Cortijo J

PA1-26204 was used in immunohistochemistry to study the role of smoking and COPD in promoting epithelial-to-mesenchymal transition in small airways

Wed May 01 00:00:00 UTC 2013

Aclidinium inhibits human lung fibroblast to myofibroblast transition.

Thorax

Milara J,Serrano A,Peiró T,Gavaldà A,Miralpeix M,Morcillo EJ,Cortijo J

Published figure using Collagen I polyclonal antibody (Product # PA1-26204) in Western Blot

Thu Mar 01 00:00:00 UTC 2012

Vitamin D inhibits proliferation and profibrotic marker expression in hepatic stellate cells and decreases thioacetamide-induced liver fibrosis in rats.

Gut

Abramovitch S,Dahan-Bachar L,Sharvit E,Weisman Y,Ben Tov A,Brazowski E,Reif S

PA1-26204 was used in western blot to study the role of vitamin D in hepatic stellate cell proliferation and liver fibrosis

Thu Dec 01 00:00:00 UTC 2011

生物信息学

蛋白别名: alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; alpha-1 type 1 collagen; Alpha-1 type I collagen; Alpha-2 type I collagen; alpha1(I) procollagen; COL1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-2(I) chain; collagen COL1A2; collagen I, alpha-2 polypeptide; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, type 1, alpha 1; collagen, type I, alpha 1; collagen, type I, alpha 2; osteogenesis imperfecta; pro-alpha-1 collagen type 1; procollagen type I, alpha 1; procollagen, type 1, alpha 1; procollagen, type I, alpha 1; procollagen, type I, alpha 2; Type I Collagen; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type I-alpha 1 collagen

查看更多收起

基因别名: AA960264; AI325291; BOS_18945; BOS_4009; Col1a-1; Col1a-2; COL1A1; COL1A2; Cola-1; Cola-2; Cola1; Cola2; COLIA1; EDSC; Mov-13; Mov13; OI1; OI2; OI3; OI4; oim

查看更多收起

功能

extracellular matrix structural constituent protein binding identical protein binding metal ion binding platelet-derived growth factor binding protein binding, bridging SMAD binding molecular_function

参与通路

Collagen I Polyclonal Antibody

Collagen I Antibody (PA1-26204) in IHC (P)

产品信息

PA1-26204

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

点杂交 (DB)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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