Invitrogen

Connexin 40 Monoclonal Antibody (2F9A11)

Connexin 40 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Connexin 40 Antibody (37-8900) in IHC (P)

Immunohistochemistry analysis of Connexin 40 showing staining in the membrane of paraffin-embedded human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temp... View More

Connexin 40 Antibody in Western Blot (WB)

Connexin 40 Antibody in Immunohistochemistry (IHC)

Connexin 40 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

产品信息

37-8900

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看7篇已发表文章 7篇已发表文章

免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫组化（冰冻） (IHC (F))

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Pig, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2F9A11

抗原

Synthetic peptide corresponding to the C-terminus of mouse Connexin40

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533346

靶标信息

Connexin 40 (Cx40), belongs to the connexin family of gap junction proteins, which are expressed in pancreas, kidney, skeletal muscle, liver, placenta and heart. Connexin 40 is highly expressed in vascular endothelial cells, and the mammalian heart (specifically in the atrial myocardium, and the His-Purkinje conduction system). At least three other connexins are expressed in the heart, including Cx37 (endocardium), Cx43 (atrial and ventricular myocardium, and distal parts of the conduction system) and Cx45 (throughout the heart). Cx40 and Cx43 frequently co-localize in cells of the cardiovascular system and participate in the formation of heterotypic gap junction channels.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Novel Blood Vascular Endothelial Subtype-Specific Markers in Human Skin Unearthed by Single-Cell Transcriptomic Profiling.

Cells

He Y,Tacconi C,Dieterich LC,Kim J,Restivo G,Gousopoulos E,Lindenblatt N,Levesque MP,Claassen M,Detmar M

37-8900 was used in Immunohistochemistry-immunofluorescence to dissect the molecular heterogeneity of blood vascular endothelial cells (BECs) in healthy adult human skin and identified six different subpopulations, signifying arterioles, post-arterial capillaries, pre-venular capillaries, post-capillary venules, venules and collecting venules.

Fri Mar 25 00:00:00 UTC 2022

Novel Blood Vascular Endothelial Subtype-Specific Markers in Human Skin Unearthed by Single-Cell Transcriptomic Profiling.

Cells

He Y,Tacconi C,Dieterich LC,Kim J,Restivo G,Gousopoulos E,Lindenblatt N,Levesque MP,Claassen M,Detmar M

Fri Mar 25 00:00:00 UTC 2022

Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing.

Nature communications

Liang D,Xue J,Geng L,Zhou L,Lv B,Zeng Q,Xiong K,Zhou H,Xie D,Zhang F,Liu J,Liu Y,Li L,Yang J,Xue Z,Chen YH

37-8900 was used in Immunohistochemistry to identify four distinct transcriptional clusters by single-cell RNA sequencing in the mouse sinoatrial node.

Tue Jan 12 00:00:00 UTC 2021

ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction.

Circulation research

Dai W,Nadadur RD,Brennan JA,Smith HL,Shen KM,Gadek M,Laforest B,Wang M,Gemel J,Li Y,Zhang J,Ziman BD,Yan J,Ai X,Beyer EC,Lakata EG,Kasthuri N,Efimov IR,Broman MT,Moskowitz IP,Shen L,Weber CR

37-8900 was used in Immunohistochemistry to determine the role of ZO-1 in cardiac function.

Fri Jul 03 00:00:00 UTC 2020

ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction.

Circulation research

Dai W,Nadadur RD,Brennan JA,Smith HL,Shen KM,Gadek M,Laforest B,Wang M,Gemel J,Li Y,Zhang J,Ziman BD,Yan J,Ai X,Beyer EC,Lakata EG,Kasthuri N,Efimov IR,Broman MT,Moskowitz IP,Shen L,Weber CR

37-8900 was used in Immunohistochemistry to determine the role of ZO-1 in cardiac function.

Fri Jul 03 00:00:00 UTC 2020

ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction.

Circulation research

Dai W,Nadadur RD,Brennan JA,Smith HL,Shen KM,Gadek M,Laforest B,Wang M,Gemel J,Li Y,Zhang J,Ziman BD,Yan J,Ai X,Beyer EC,Lakata EG,Kasthuri N,Efimov IR,Broman MT,Moskowitz IP,Shen L,Weber CR

37-8900 was used in Immunohistochemistry to determine the role of ZO-1 in cardiac function.

Fri Jul 03 00:00:00 UTC 2020

ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction.

Circulation research

Dai W,Nadadur RD,Brennan JA,Smith HL,Shen KM,Gadek M,Laforest B,Wang M,Gemel J,Li Y,Zhang J,Ziman BD,Yan J,Ai X,Beyer EC,Lakata EG,Kasthuri N,Efimov IR,Broman MT,Moskowitz IP,Shen L,Weber CR

37-8900 was used in Immunohistochemistry to determine the role of ZO-1 in cardiac function.

Fri Jul 03 00:00:00 UTC 2020

In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix.

Nature communications

Lee J,Sutani A,Kaneko R,Takeuchi J,Sasano T,Kohda T,Ihara K,Takahashi K,Yamazoe M,Morio T,Furukawa T,Ishino F

37-8900 was used in Immunocytochemistry to demonstrate that this method not only provides a biomimetic model of the developing heart-like structure with simplified differentiation protocol, but also represents a promising research tool with a broad range of applications, including drug testing.

Thu Sep 03 00:00:00 UTC 2020

In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix.

Nature communications

Lee J,Sutani A,Kaneko R,Takeuchi J,Sasano T,Kohda T,Ihara K,Takahashi K,Yamazoe M,Morio T,Furukawa T,Ishino F

Thu Sep 03 00:00:00 UTC 2020

Probing Endothelial Cell Mechanics Through Connexin 43 Disruption.

Experimental mechanics

Islam MM,Steward RL

37-8900 was used in Immunohistochemistry to investigated the role Cx43 plays in endothelial cell intercellular stress generation and traction generation to bring clarity to the relationship between Cx43 and endothelial cell mechanics.

Fri Mar 01 00:00:00 UTC 2019

Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.

Nature communications

Baron CS,Kester L,Klaus A,Boisset JC,Thambyrajah R,Yvernogeau L,Kouskoff V,Lacaud G,van Oudenaarden A,Robin C

37-8900 was used in Immunohistochemistry on frozen tissues to study the molecular events controlling endothelial specification, endothelial-to-haematopoietic transition, and intra-aortic haematopoietic cluster formation, as it occurs in vivo inside the aorta.

Thu Jun 28 00:00:00 UTC 2018

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

The European journal of neuroscience

Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI

37-8900 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus

Sat Mar 01 00:00:00 UTC 2014

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

The European journal of neuroscience

Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI

37-8900 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus

Sat Mar 01 00:00:00 UTC 2014

Connexins and M3 muscarinic receptors contribute to heterogeneous Ca(2+) signaling in mouse aortic endothelium.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Boittin FX,Alonso F,Le Gal L,Allagnat F,Bény JL,Haefliger JA

37-8900 was used in immunohistochemistry to examine the role of connexin 40 in the endothelial Ca(2+) signaling of the mouse aorta.

Thu Sep 05 00:00:00 UTC 2013

Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.

Cell communication & adhesion