Invitrogen

Cyclin E Monoclonal Antibody (HE12)

Cyclin E Antibody in Immunocytochemistry (ICC/IF)

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Cyclin E Antibody (32-1600) in ICC/IF

Immunofluorescent analysis of Cyclin E was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Cyclin E Mouse Monoclonal Antibody (Product # 32-1600) at 2 µg - 4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:40... View More

Cyclin E Antibody in Immunohistochemistry (IHC)

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产品信息

32-1600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

查看11篇已发表文章 11篇已发表文章

免疫组化 (IHC)

Assay-dependent

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1 µg

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HE12

抗原

Full-length, recombinant, human cyclin E protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533067

靶标信息

Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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