Rockland

Cyclin E2 Polyclonal Antibody

图: 1 / 1

Cyclin E2 Antibody (600-401-971) in WB

Western blot using Rocklands affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD.

Product Image Gallery

产品信息

600-401-971

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:2,000

酶联免疫吸附实验 (ELISA)

1:20,000-1:85,000

免疫沉淀 (IP)

1:100

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at the carboxyl terminus of the Cyclin E2 protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.17 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against mouse Cyclin E2 protein. A BLAST analysis was used to suggest cross-reactivity with Cyclin E2 protein from human based on 100% homology with the immunizing sequence. Cross-reactivity with Cyclin E2 from rat is also predicted based on a 91% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

靶标信息

This gene encodes WW domain-containing binding protein 4. The WW domain represents a small and compact globular structure that interacts with proline-rich ligands. This encoded protein is a general spliceosomal protein that may play a role in cross-intron bridging of U1 and U2 snRNPs in the spliceosomal complex A.

仅用于科研。不用于诊断过程。未经明确授权不得转售。