Rockland

Cyclin L1 Polyclonal Antibody

产品信息

200-401-881

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:5,000

免疫组化 (IHC)

10-20 µg/mL

酶联免疫吸附实验 (ELISA)

1:5,000-1:25,000

免疫沉淀 (IP)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to an internal region near amino acids 300-375 of Human Cyclin L1a protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

5 mg/mL

纯化类型

Protein A

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96% homology with the protein from these sources. Cross reactivity with Cyclin L1a homologues from other sources has not been determined.

靶标信息

FUS/TLS was originally described as fused in Sarcoma is actually related to a number of different cellular processes and correlated to several diseases. FUS binds DNA as a single or double strand, and acts as a site for transcription factors to recognize. Since FUS is well-documented to be involved in liposarcoma, lipoma and leukaemia, it is believed that when its normal housekeeping functions related to transcription are disrupted, normal cells become cancerous. Interestingly, FUS is also associated with the fatal neuron disorder amyotrophic lateral sclerosis; interruption of its normal RNA-binding functions leads to incorrect cellular localization and leads to pathologies of the neuron. Thus, FUS regulation of cellular functions is a critical component of maintenance of healthy cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。