Invitrogen

DLL1 (delta-like 1) Monoclonal Antibody (HMD1-5), Alexa Fluor™ 488, eBioscience™

DLL1 (delta-like 1) Antibody in Flow Cytometry (Flow)

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DLL1 (delta-like 1) Antibody (53-5767-82) in Flow

Staining of CD8+CD11c+ gated C57BL/6 splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control Alexa Fluor® 488 (Product # 53-4888-80) (blue histogram) or 0.5 µg of Anti-Mouse DLL1 (delta-like 1) Alexa Fluor® 488 (purple histogram). Total viable cells were used for analysis.

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产品信息

53-5767-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

HMD1-5

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1548650

产品详细信息

Description: The HMD1-5 monoclonal antibody reacts with mouse Delta-like 1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Delta-like 1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Delta-like 1 is expressed by thymic stromal cells, as well as on macrophages, dendritic cells, and stromal cells in the spleen. This protein is also expressed in nonhematopoietic tissues such as the lung and brain. Delta-like 1 has been linked to B cell development and differentiation, especially marginal zone and plasma cells, regulation of splenic dendritic cells, and leukemogenesis.

Applications Reported: This HMD1-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HMD1-5 antibody has been tested by flow cytometric analysis on mouse splenocytes and transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Delta-like protein 1 (DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。