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DNase II Antibody (PA5-115136) in ICC/IF
Immunocytochemistry analysis of DNase II in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115136) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115136
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human DNASE2(Accession O00115), corresponding to amino acid residues E209-T259.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899772
产品详细信息
Antibody detects endogenous levels of total DNase II.
靶标信息
DNase II is a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Acid DNase; deoxyribonuclease; Deoxyribonuclease II alpha; Deoxyribonuclease-2-alpha; DNase II alpha; DNase II, lysosomal; Lysosomal DNase II; R31240_2
基因别名: DNASE2; DNASE2A; DNL; DNL2
UniProt ID: (Human) O00115, (Rat) Q9QZK8
Entrez Gene ID: (Human) 1777, (Rat) 171575
