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Doublecortin Antibody (600-101-MH8) in ICC/IF
Immunofluorescence microscopy of Anti-Doublecortin in PND1 cells. Post-natal rat pup (PND1) heterogeneous brain cells were fixed with 4% PFA, permeabilization 0.3% Triton X-100. Incubated with 15µg/mL of anti-Doublecortin primary antibody at 4°C overnight. Counterstain Donkey anti-Goat IgG Dylight™488 Conjugated antibody (p/n 605-741-125) at 5µg/mL for 1hr at room temperature. Nuclei were counterstained wit... View More
产品信息
600-101-MH8
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-Doublecortin antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human doublecortin conjugated to Keyhole Limpet Hemocyanin (KLH).
偶联物
形式
Liquid
浓度
0.79 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human Neuronal migration protein doublecortin. Blast analysis the immunogen sequence shows 100% identity withrat.
靶标信息
In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; lissencephaly, X-linked (doublecortin); Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316
基因别名: DBCN; Dbct; DC; Dcn; DCX; LISX; SCLH; XLIS
UniProt ID: (Human) O43602, (Mouse) O88809
Entrez Gene ID: (Human) 1641, (Rat) 84394, (Mouse) 13193
neuron migration
brain development
central nervous system projection neuron axonogenesis
intracellular signal transduction
axon extension
dendrite morphogenesis
response to electrical stimulus
multicellular organism development
nervous system development
peptidyl-serine phosphorylation
cell differentiation
protein autophosphorylation
central nervous system development
hippocampus development
layer formation in cerebral cortex
pyramidal neuron development
