Invitrogen

Doublecortin Polyclonal Antibody

Doublecortin Antibody in Immunocytochemistry (ICC/IF)

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Doublecortin Antibody (PA5-17428) in ICC/IF

Immunofluorescence analysis of Doublecortin was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Doublecortin Rabbit Polyclonal Antibody (Product # PA5-17428) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and... View More

Doublecortin Antibody in Western Blot (WB)

Doublecortin Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-17428

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:200-1:800

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to human doublecortin

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

130 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10977233

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. DCX is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. It contains two doublecortin domains, which bind microtubules. In addition, this protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in the gene encoding DCX are a cause of X-linked lissencephaly.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Enriched Environment Induces Sex-Specific Changes in the Adult Neurogenesis, Cytokine and miRNA Expression in Rat Hippocampus.

Biomedicines

Vinogradova A,Sysova M,Smirnova P,Sidorova M,Turkin A,Kurilova E,Tuchina O

PA5-17428 was used in Immunohistochemistry to study the behavior and hippocampal neurogenesis in adult male and female Wistar rats that were housed in an enriched environment (EE) for two months. Both EE males and females performed better than control animals in a Barnes maze, meaning that EE enhances spatial memory.

Tue May 02 00:00:00 UTC 2023

Extracellular vesicles from neural progenitor cells promote functional recovery after stroke in mice with pharmacological inhibition of neurogenesis.

Cell death discovery

Campero-Romero AN,Real FH,Santana-Martínez RA,Molina-Villa T,Aranda C,Ríos-Castro E,Tovar-Y-Romo LB

PA5-17428 was used in Immunocytochemistry-immunofluorescence to indicate that NPC-derived EVs are protective against the ischemic cascade activated by stroke and, thus, hold significant therapeutic potential.

Fri Jul 28 00:00:00 UTC 2023

Prenatal glucocorticoid exposure selectively impairs neuroligin 1-dependent neurogenesis by suppressing astrocytic FGF2-neuronal FGFR1 axis.

Cellular and molecular life sciences : CMLS

Choi GE,Chae CW,Park MR,Yoon JH,Jung YH,Lee HJ,Han HJ

PA5-17428 was used in Immunohistochemistry to identify neuroligin 1 and its regulator INFS as potential targets for treating prenatal glucocorticoid-induced neurogenesis defects and memory/mood disorders.

Fri May 13 00:00:00 UTC 2022

Premature Neural Progenitor Cell Differentiation Into Astrocytes in Retinoic Acid-Induced Spina Bifida Rat Model.

Frontiers in molecular neuroscience

Oria M,Pathak B,Li Z,Bakri K,Gouwens K,Varela MF,Lampe K,Murphy KP,Lin CY,Peiro JL

PA5-17428 was used in Immunohistochemistry to show that changes in NPC patterning factor expression with Notch-BMP signaling upregulation may be responsible for the altered astrogenesis patterns observed in the spinal cord in a retinoic acid-induced spina bifida model.

Tue Jan 24 00:00:00 UTC 2023

Premature Neural Progenitor Cell Differentiation Into Astrocytes in Retinoic Acid-Induced Spina Bifida Rat Model.

Frontiers in molecular neuroscience

Oria M,Pathak B,Li Z,Bakri K,Gouwens K,Varela MF,Lampe K,Murphy KP,Lin CY,Peiro JL

Tue Jan 24 00:00:00 UTC 2023

生物信息学

蛋白别名: doublecortex; Doublin; Lis-X; LIS-XD; Lissencephalin-X; lissencephaly, X-linked (doublecortin); Neuronal migration protein doublecortin; OTTHUMP00000023860; OTTHUMP00000216316

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基因别名: DBCN; Dbct; DC; Dcn; DCX; LISX; SCLH; XLIS

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UniProt ID: (Human) O43602, (Mouse) O88809

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Entrez Gene ID: (Human) 1641, (Rat) 84394, (Mouse) 13193

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功能

calmodulin-dependent protein kinase activity protein binding calmodulin binding microtubule binding calcium-dependent protein serine/threonine kinase activity protein kinase binding

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