Invitrogen

Dynamin 2 Polyclonal Antibody

Dynamin 2 Antibody in Immunocytochemistry (ICC/IF)

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Dynamin 2 Antibody (PA1-661) in ICC/IF

Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody (Product # PA1-661) at 1:100 in 0.1% BSA, incubated at 4 degree celsius over... View More

Dynamin 2 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-661

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

查看14篇已发表文章 14篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C S(760) P T P Q R R P V S S I H P P G R P P A(779)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2293040

产品详细信息

PA1-661 detects dynamin II (Dyn2) from rat, mouse, and human tissues and cells.

PA1-661 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn2 from HeLa cell lysate.

PA1-661 immunizing peptide corresponds to amino acid residues 760-779 from human Dyn2. This sequence is 95% conserved in mouse and rat Dyn2. PA1-661 immunizing peptide (Cat. # PEP-056) is available for use in neutralization and control experiments.

靶标信息

Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Five alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Impairment of Autophagic Flux After Hypobaric Hypoxia Potentiates Oxidative Stress and Cognitive Function Disturbances in Mice.

Neuroscience bulletin

Dai S,Feng Y,Lu C,Zhang H,Ma W,Xie W,Wu X,Luo P,Zhang L,Fei F,Fei Z,Li X

PA1-661 was used in Immunohistochemistry (Paraffin) to investigate the role of autophagy in hypobaric hypoxia (HH).

Mon Jan 01 00:00:00 UTC 2024

Impairment of Autophagic Flux After Hypobaric Hypoxia Potentiates Oxidative Stress and Cognitive Function Disturbances in Mice.

Neuroscience bulletin

Dai S,Feng Y,Lu C,Zhang H,Ma W,Xie W,Wu X,Luo P,Zhang L,Fei F,Fei Z,Li X

PA1-661 was used in Immunohistochemistry (Paraffin) to investigate the role of autophagy in hypobaric hypoxia (HH).

Mon Jan 01 00:00:00 UTC 2024

Dynamin2 functions as an accessory protein to reduce the rate of caveola internalization.

The Journal of cell biology

Larsson E,Morén B,McMahon KA,Parton RG,Lundmark R

PA1-661 was used in Western Blotting to propose that Dyn2 is not a core component of the caveolae machinery, but rather functions as an accessory protein that restrains caveola internalization.

Mon Apr 03 00:00:00 UTC 2023

Dynamin2 functions as an accessory protein to reduce the rate of caveola internalization.

The Journal of cell biology

Larsson E,Morén B,McMahon KA,Parton RG,Lundmark R

PA1-661 was used in Western Blotting to propose that Dyn2 is not a core component of the caveolae machinery, but rather functions as an accessory protein that restrains caveola internalization.

Mon Apr 03 00:00:00 UTC 2023

Efficient Protein Transfection by Swarms of Chemically Powered Plasmonic Virus-Sized Nanorobots.

ACS nano

Ressnerova A,Novotny F,Michalkova H,Pumera M,Adam V,Heger Z

PA1-661 was used in Immunocytochemistry-immunoflourescence to show that protein transfection mediated by such nanorobots is an important step forward, challenging the field of nanomedicine and having potential in future translational medical research.

Tue Aug 24 00:00:00 UTC 2021

Identification of host proteins differentially associated with HIV-1 RNA splice variants.

eLife

Knoener R,Evans E,Becker JT,Scalf M,Benner B,Sherer NM,Smith LM

PA1-661 was used in Western Blot, Immunocytochemistry to validate HyPR-MSSV for discovery of viral splice variant protein interactomes and provides an unprecedented resource of factors and pathways likely important to HIV-1 replication.

Thu Feb 25 00:00:00 UTC 2021

Identification of host proteins differentially associated with HIV-1 RNA splice variants.

eLife

Knoener R,Evans E,Becker JT,Scalf M,Benner B,Sherer NM,Smith LM

Thu Feb 25 00:00:00 UTC 2021

The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina.

Cell reports

Toulis V,García-Monclús S,de la Peña-Ramírez C,Arenas-Galnares R,Abril JF,Todi SV,Khan N,Garanto A,Costa MDC,Marfany G

PA1-661 was used in Immunohistochemistry to unveil the function of Ataxin-3 in the retina.

Tue Nov 10 00:00:00 UTC 2020

JC Polyomavirus Entry by Clathrin-Mediated Endocytosis Is Driven by β-Arrestin.

Journal of virology

Mayberry CL,Soucy AN,Lajoie CR,DuShane JK,Maginnis MS

PA1-661 was used in Western Blotting to characterize how JCPyV utilizes the clathrin-mediated endocytosis pathway to invade host cells.

Mon Apr 15 00:00:00 UTC 2019

SNX18 regulates ATG9A trafficking from recycling endosomes by recruiting Dynamin-2.

EMBO reports

Søreng K,Munson MJ,Lamb CA,Bjørndal GT,Pankiv S,Carlsson SR,Tooze SA,Simonsen A

Published figure using Dynamin 2 polyclonal antibody (Product # PA1-661) in Western Blot

Sun Apr 01 00:00:00 UTC 2018

Small molecule induced oligomerization, clustering and clathrin-independent endocytosis of the dopamine transporter.

eLife

Sorkina T,Ma S,Larsen MB,Watkins SC,Sorkin A

PA1-661 was used in Western Blotting to suggest that AIM-100 augments dopamine transporters (DAT) oligomerization through an allosteric mechanism associated with the DAT conformational state.

Mon Apr 09 00:00:00 UTC 2018

Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells.

Beilstein journal of nanotechnology

Bohmer N,Jordan A

PA1-661 was used in Western Blotting to demonstrate that Caveolin-1 and CDC42 mediate the endocytosis of silica-coated iron oxide nanoparticles in HeLa cells in vitro.

Wed Feb 11 00:00:00 UTC 2015

Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.

mBio

Olofsson A,Nygård Skalman L,Obi I,Lundmark R,Arnqvist A

PA1-661 was used in western blot to study the role of clathrin-dependent and clathrin-independent routes in the uptake of Helicobacter pylori vesicles by gastric epithelial cells

Tue May 20 00:00:00 UTC 2014

Protein partners of dynamin-1 in the retina.

Visual neuroscience

Grossman GH,Ebke LA,Beight CD,Jang GF,Crabb JW,Hagstrom SA

PA1-661 was used in immunohistochemistry and western blot to identify protein binding partners of retinal dynamin-1

Mon Jul 01 00:00:00 UTC 2013

Protein partners of dynamin-1 in the retina.

Visual neuroscience

Grossman GH,Ebke LA,Beight CD,Jang GF,Crabb JW,Hagstrom SA

PA1-661 was used in immunohistochemistry and western blot to identify protein binding partners of retinal dynamin-1

Mon Jul 01 00:00:00 UTC 2013

Induction of the alternative NF-κB pathway by lymphotoxin αβ (LTαβ) relies on internalization of LTβ receptor.

Molecular and cellular biology

Ganeff C,Remouchamps C,Boutaffala L,Benezech C,Galopin G,Vandepaer S,Bouillenne F,Ormenese S,Chariot A,Schneider P,Caamaño J,Piette J,Dejardin E

PA1-661 was used in western blot to study the mechanism by which the alternative NF-kappaB pathway is induced by lymphotoxin alphabeta

Tue Nov 01 00:00:00 UTC 2011

Dynamin 2 is required for the enhancement of HIV-1 infectivity by Nef.

Proceedings of the National Academy of Sciences of the United States of America

Pizzato M,Helander A,Popova E,Calistri A,Zamborlini A,Palù G,Göttlinger HG

PA1-661 was used in western blot to study to the role of dynamin 2 in HIV-1 infectivity.

Tue Apr 17 00:00:00 UTC 2007

Beta-amyloid-induced dynamin 1 depletion in hippocampal neurons. A potential mechanism for early cognitive decline in Alzheimer disease.

The Journal of biological chemistry

Kelly BL,Vassar R,Ferreira A

PA1-661 was used in western blot to demonstrate a potential mechanism for early cognitive decline in Alzheimer disease.

Fri Sep 09 00:00:00 UTC 2005

Detergent-resistant membrane microdomains facilitate Ib oligomer formation and biological activity of Clostridium perfringens iota-toxin.

Infection and immunity

Hale ML,Marvaud JC,Popoff MR,Stiles BG

PA1-661 was used in western blot to locate iota b (Ib) oligomers in detergent-resistant membrane microdomains (DRMs) extracted from Vero cells

Thu Apr 01 00:00:00 UTC 2004

Analysis of clathrin-mediated endocytosis of epidermal growth factor receptor by RNA interference.

The Journal of biological chemistry

Huang F,Khvorova A,Marshall W,Sorkin A

PA1-661 was used in western blot to assess the importance of clathrin, AP-2, and other endocytic proteins for EGFR endocytosis.

Fri Apr 16 00:00:00 UTC 2004

Aquaporin-2 localization in clathrin-coated pits: inhibition of endocytosis by dominant-negative dynamin.

American journal of physiology. Renal physiology

Sun TX,Van Hoek A,Huang Y,Bouley R,McLaughlin M,Brown D

PA1-661 was used in immunocytochemistry to investigate the aquaporin-2 localization in clathrin-coated pits.

Sat Jun 01 00:00:00 UTC 2002

生物信息学

蛋白别名: Dynamin 2; Dynamin II; Dynamin UDNM; Dynamin-2

查看更多收起

基因别名: b2b2159Clo; CMT2M; CMTDI1; CMTDIB; DI-CMTB; DNM2; DYIIAAB; DYN2; DYNII; LCCS5; Udnm

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UniProt ID: (Human) P50570, (Rat) P39052, (Mouse) P39054

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Entrez Gene ID: (Human) 1785, (Rat) 25751, (Mouse) 13430

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功能

GTPase activity protein binding GTP binding microtubule binding SH3 domain binding enzyme binding protein kinase binding D2 dopamine receptor binding protein complex binding phosphatidylinositol 3-kinase regulatory subunit binding WW domain binding nitric-oxide synthase binding nucleotide binding hydrolase activity membrane traffic protein

参与通路

Dynamin 2 Polyclonal Antibody

Dynamin 2 Antibody (PA1-661) in ICC/IF

产品信息

PA1-661

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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