Invitrogen

E-cadherin Polyclonal Antibody

E-cadherin Antibody in Immunocytochemistry (ICC/IF)

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E-cadherin Antibody (PA5-32178) in ICC/IF

Immunofluorescence analysis of E-cadherin was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with E-cadherin Polyclonal Antibody (Product # PA5-32178) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ce... View More

E-cadherin Antibody in Western Blot (WB)

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产品信息

PA5-32178

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

查看9篇已发表文章 9篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Rat

已发表种属

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 338 and 649 of Human E-cadherin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.09 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2549651

产品详细信息

Recommended positive controls: 293T, PC-12, MCF-7.

Predicted reactivity: Rat (80%), Pig (82%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An immunoresponsive three-dimensional urine-tolerant human urothelial model to study urinary tract infection.

Frontiers in cellular and infection microbiology

Jafari NV,Rohn JL

PA5-32178 was used in Immunocytochemistry to develop a human urothelial three-dimensional (3D) model which was urine tolerant and demonstrated several urothelial biomarkers, but it only achieved human thickness in heterogenous, multi-layered zones and did not demonstrate the comprehensive differentiation status needed to achieve barrier function.

Fri Apr 14 00:00:00 UTC 2023

Development of rat duodenal monolayer model with effective barrier function from rat organoids for ADME assay.

Scientific reports

Tanaka K,Kawai S,Fujii E,Yano M,Miyayama T,Nakano K,Terao K,Suzuki M

PA5-32178 was used in Immunohistochemistry to expect our novel monolayer model to be a useful tool for elucidating drug absorption processes in detail, enabling the development of highly absorbable drugs.

Wed Jul 26 00:00:00 UTC 2023

Re-expression of circ_0043610 contributes to trophoblast dysfunction through the miR-558/RYBP pathway in preeclampsia.

Endocrine journal

Shang J,Lin L,Huang X,Zhou L,Huang Q

PA5-32178 was used in Western Blotting to investigate the function and the potential mechanism of circ_0043610 in preeclampsia.

Wed Dec 28 00:00:00 UTC 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

PA5-32178 was used in Western Blotting to support that betulonic acid inhibits the invasion and metastasis of gastric cancer cells by mediating cytoskeletal remodeling and regulating epithelial mesenchymal transformation.

Wed Feb 02 00:00:00 UTC 2022

Betulonic Acid, as One of the Active Components of the Celastrus orbiculatus Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.

Molecules (Basel, Switzerland)

Chu Z,Luo Y,Ni T,Zhu M,Feng X,Liu Y,Wang H

Wed Feb 02 00:00:00 UTC 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

PA5-32178 was used in Western Blotting to conclude that CircATXN7 accelerated the malignancy of NSCLC cells through adsorbing miR-7-5p and upregulating PFN2, offering evidence to support circATXN7 as a target for NSCLC treatment.

Wed Jun 01 00:00:00 UTC 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

Wed Jun 01 00:00:00 UTC 2022

Poria Acid, Triterpenoids Extracted from Poria cocos, Inhibits the Invasion and Metastasis of Gastric Cancer Cells.

Molecules (Basel, Switzerland)

Wang H,Luo Y,Chu Z,Ni T,Ou S,Dai X,Zhang X,Liu Y

PA5-32178 was used in Western Blotting to show that Triterpenoids from P. cocos have significant biological activity against gastric cancer, and the mechanism may be involved in the process of epithelial-mesenchymal transformation.

Mon Jun 06 00:00:00 UTC 2022

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-32178 was used in Western Blot, Immunocytochemistry to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 UTC 2021

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-32178 was used in Western Blot, Immunocytochemistry to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 UTC 2021

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-32178 was used in Western Blot, Immunocytochemistry to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 UTC 2021

RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers.

Frontiers in oncology

Abraham HG,Ulintz PJ,Goo L,Yates JA,Little AC,Bao L,Wu Z,Merajver SD

PA5-32178 was used in Western Blot, Immunohistochemistry to study how RhoA- and RhoC-GTPase influence the cell-cell junctions in aggressive breast cancers.

Tue Nov 07 00:00:00 UTC 2023

RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers.

Frontiers in oncology

Abraham HG,Ulintz PJ,Goo L,Yates JA,Little AC,Bao L,Wu Z,Merajver SD

PA5-32178 was used in Western Blot, Immunohistochemistry to study how RhoA- and RhoC-GTPase influence the cell-cell junctions in aggressive breast cancers.

Tue Nov 07 00:00:00 UTC 2023

E2F5 Promotes the Malignancy of Ovarian Cancer Via the Regulation of Hippo and Wnt Pathways.

Genetic testing and molecular biomarkers

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-32178 was used in Western Blot to conclude that E2F5 promotes ovarian carcinogenesis via the regulation of Hippo and Wnt pathways.

Mon Mar 01 00:00:00 UTC 2021

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-32178 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 UTC 2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

BMC cancer

Malgundkar SH,Burney I,Al Moundhri M,Al Kalbani M,Lakhtakia R,Okamoto A,Tamimi Y

PA5-32178 was used in Western Blotting to examine the role of FAT4 in proliferation and metastasis, and its mechanisms of interaction in these processes.

Mon May 04 00:00:00 UTC 2020

Monitoring Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction.

Methods and protocols

Sim JJ,Jeong KY

PA5-32178 was used in Immunocytochemistry-immunoflourescence to investigate mitochondrial dysfunction following epithelial-mesenchymal transition in pancreatic cancer cells in an hypoxic environment and ensure their correlation.

Mon Apr 27 00:00:00 UTC 2020

Monitoring Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells via Investigation of Mitochondrial Dysfunction.

Methods and protocols

Sim JJ,Jeong KY

Mon Apr 27 00:00:00 UTC 2020

Biophysical Control of Bile Duct Epithelial Morphogenesis in Natural and Synthetic Scaffolds.

Frontiers in bioengineering and biotechnology

Funfak A,Bouzhir L,Gontran E,Minier N,Dupuis-Williams P,Gobaa S

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Immunocytochemistry

Wed Sep 30 00:00:00 UTC 2020

Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.

International journal of molecular sciences

Kao HH,Kuo CY,Chen KS,Chen JP

PA5-32178 was used in Immunohistochemistry-immunofluorescence to explore the possibility to culture mesothelial cells in a three-dimensional scaffold for tissue engineering applications.

Thu Sep 12 00:00:00 UTC 2019

Secreted Giardia intestinalis cysteine proteases disrupt intestinal epithelial cell junctional complexes and degrade chemokines.

Virulence

Liu J,Ma'ayeh S,Peirasmaki D,Lundström-Stadelmann B,Hellman L,Svärd SG

Published figure using E-cadherin polyclonal antibody (Product # PA5-32178) in Western Blot

Mon Dec 31 00:00:00 UTC 2018

Tumor bioengineering using a transglutaminase crosslinked hydrogel.

PloS one

Fang JY,Tan SJ,Yang Z,Tayag C,Han B

PA5-32178 was used in immunohistochemistry to develop a 3D culturing system to mimic the tumor microenvironment using transglutaminase-cross-linked gelatin gel (Col-Tgel)

Mon Dec 07 00:00:00 UTC 2015

生物信息学

蛋白别名: cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin

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基因别名: Arc-1; CD324; CDH1; CDHE; ECAD; LCAM; UVO

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UniProt ID: (Human) P12830, (Rat) Q9R0T4

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Entrez Gene ID: (Human) 999, (Rat) 83502

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功能