Invitrogen

EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

EGFR Antibody in Immunocytochemistry (ICC/IF)

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EGFR Antibody (700308) in ICC/IF

Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGFR Antibody (30H45L48), Recombinant Rabbit Monoclonal (Product # 700308) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488... View More

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EGFR Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. A higher level of expression of EGFR was observed in A-431 compared to A549, HeLa, U-87 MG and HCT116 using EGFR Monoclonal Antibody (Product # 700308) in Western Blot. {RE}

产品信息

700308

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

5 µg/mL

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染色质免疫沉淀实验 (ChIP)

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产品规格

种属反应

Human

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

30H45L48

抗原

proprietary

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532314

产品详细信息

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

700308 was used in Immunocytochemistry and Western Blotting to develop a new model of epithelial growth factor receptor internalization and degradation upon erlotinib treatment in pancreatic ductal adenocarcinoma cells cultured in a 3D self-assembling peptide scaffold.

Tue Sep 07 00:00:00 UTC 2021

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

Tue Sep 07 00:00:00 UTC 2021

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

Tue Sep 07 00:00:00 UTC 2021

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

Tue Sep 07 00:00:00 UTC 2021

VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia.

Brain sciences

Peña JS,Vazquez M

700308 was used in Immunocytochemistry to show an interplay between VEGF and EGF-R pathways in Müller glia.

Fri May 29 00:00:00 UTC 2020

Multiplexed Analysis for Anti-Epidermal Growth Factor Receptor Tumor Cell Growth Inhibition Based on Quantum Dot Probes.

Analytical chemistry

Ren D,Xia Y,Wang B,You Z

700308 was used in immunocytochemistry to describe a multiplex analysis method to examine anti-EGFR functions in heterogeneous cells

Tue Apr 19 00:00:00 UTC 2016

Functional evaluation of fluorescein-labeled derivatives of a peptide inhibitor of the EGF receptor dimerization.

Bioorganic & medicinal chemistry

Toyama K,Mizuguchi T,Nomura W,Tamamura H

700308 was used in western blot to characterize novel EGF receptor inhibitors

Mon Aug 15 00:00:00 UTC 2016

EGF as a New Therapeutic Target for Medulloblastoma Metastasis.

Cellular and molecular bioengineering

Rico-Varela J,Singh T,McCutcheon S,Vazquez M

700308 was used in immunocytochemistry to examine the role of epidermal growth factor in medulloblastoma chemotactic migration

Tue Dec 01 00:00:00 UTC 2015

Detachment of cells adhered on the photoreactive phospholipid polymer surface by photoirradiation and their functionality.

Colloids and surfaces. B, Biointerfaces

Byambaa B,Konno T,Ishihara K

700308 was used in immunocytochemistry to study the utility of a photoreactive phospolipid bilayer for modulating cell adhesion and detachment and the utility for cellular engineering

Fri Mar 01 00:00:00 UTC 2013

Detachment of cells adhered on the photoreactive phospholipid polymer surface by photoirradiation and their functionality.

Colloids and surfaces. B, Biointerfaces

Byambaa B,Konno T,Ishihara K

700308 was used in immunocytochemistry to study the utility of a photoreactive phospolipid bilayer for modulating cell adhesion and detachment and the utility for cellular engineering

Fri Mar 01 00:00:00 UTC 2013

Single-nucleotide polymorphisms associated with symptomatic infection and differential human gene expression in healthy seropositive persons each implicate the cytoskeleton, integrin signaling, and oncosuppression in the pathogenesis of human parvovirus B19 infection.

The Journal of infectious diseases

Kerr JR,Kaushik N,Fear D,Baldwin DA,Nuwaysir EF,Adcock IM

700308 was used in ChIP Assay, Immunocytochemistry, Western Blot to further examine the role of the host response to parvovirus B19 in the development of symptoms and consequences of viral persistence.

Fri Jul 15 00:00:00 UTC 2005

Genome-wide analysis of allele-specific gene expression using oligo microarrays.

Methods in molecular biology (Clifton, N.J.)

Lee MP

700308 was used in Chromatin immunoprecipitation to perform genome-wide analysis of allele-specific gene expression finding that variation in gene expression is fairly common in humans.

Fri Nov 04 00:00:00 UTC 2005

生物信息学

蛋白别名: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone

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基因别名: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956

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功能

chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion transmembrane signal receptor

参与通路

EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

EGFR Antibody (700308) in ICC/IF

产品信息

700308

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

Expression System

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

EGFR Antibody (700308) in ICC/IF

产品信息

700308

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

Expression System

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)