Invitrogen

EHD2 Polyclonal Antibody

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EHD2 Antibody (PA5-116554) in WB

Western blot analysis of EHD2 in extracts of various cell lines. Samples were incubated with EHD2 Polyclonal antibody (Product # PA5-116554) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

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产品信息

PA5-116554

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human EHD2 (NP_0554162)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2901185

产品详细信息

Positive Samples: A-549, U-87MG, HeLa, Mouse lung, Rat lung

Immunogen sequence: VGTPEVLRVY IGSFWSQPLL VPDNRRLFEL EEQDLFRDIQ GLPRHAALRK LNDLVKRARL VRVHAYIISY LKKEMPSVFG KENKKKQLIL KLPVIFAKIQ LEHHISPGDF PDCQKMQELL MAHDFTKFHS LKPKLLEALD EMLTHDIAKL MPLLRQEELE STEVGVQGGA FEGTHMGPFV ERGPDEAMED GEEGSDDEAE WVVTKDKSKY DEIFYNLAPA DGKLSGSKAK TWMVGTKLPN SVLGRIWKLS DVDRDGMLDD EEFALASHLI EAKLEGHGLP ANLPRRLVPP SKRRHKGSAE

靶标信息

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。