Invitrogen

EphA2 Monoclonal Antibody (1C11A12)

EphA2 Antibody in Immunocytochemistry (ICC/IF)

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EphA2 Antibody (37-4400) in ICC/IF

Immunofluorescent analysis of EphA2 was performed using 70% confluent log phase A549 cells treated with UV-302 nm for 60 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EphA2 (1C11A12) Mouse Monoclonal Antibody (Product # 37-4400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Ale... View More

EphA2 Antibody in Immunohistochemistry (IHC)

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EphA2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of EphA2 using EphA2 monoclonal antibody (Product # 37-4400), shows induction of EphA2 expression in the cytoplasm of A549 cells upon UV treatment. {TM}

产品信息

37-4400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

2 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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免疫沉淀 (IP)

Assay-dependent

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1C11A12

抗原

Synthetic peptide derived from the C-terminal region of the human EphA2 Receptor protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533318

靶标信息

EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

MiR-210 promotes bone formation in ovariectomized rats by regulating osteogenic/adipogenic differentiation of bone marrow mesenchymal stem cells through downregulation of EPHA2.

Journal of orthopaedic surgery and research

Ren L,Zhu X,Tan J,Lv X,Wang J,Hua F

37-4400 was used in Western Blot to show that miR-210 could target and inhibit the expression of EPHA2 in BMSCs, thus promoting osteogenic differentiation and inhibiting adipogenic differentiation of BMSCs.

Mon Oct 30 00:00:00 UTC 2023

MiR-210 promotes bone formation in ovariectomized rats by regulating osteogenic/adipogenic differentiation of bone marrow mesenchymal stem cells through downregulation of EPHA2.

Journal of orthopaedic surgery and research

Ren L,Zhu X,Tan J,Lv X,Wang J,Hua F

Mon Oct 30 00:00:00 UTC 2023

Targeting Host Tyrosine Kinase Receptor EPHA2 Signaling Affects Uropathogen Infection in Human Bladder Epithelial Cells.

Pathogens (Basel, Switzerland)

Prakash PS,Kruse A,Vogel C,Schagdarsurengin U,Wagenlehner F

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Western Blot

Wed Oct 12 00:00:00 UTC 2022

Targefrin: A Potent Agent Targeting the Ligand Binding Domain of EphA2.

Journal of medicinal chemistry

Baggio C,Udompholkul P,Gambini L,Pellecchia M

37-4400 was used in Cell Culture to report on a novel agent, targefrin, that binds to EphA2-LBD with a 21 nM dissociation constant by isothermal titration calorimetry and presents an IC50 value of 10.8 nM in a biochemical assay.

Thu Nov 24 00:00:00 UTC 2022

EPHA2 Interacts with DNA-PK_cs in Cell Nucleus and Controls Ionizing Radiation Responses in Non-Small Cell Lung Cancer Cells.

Cancers

Kaminskyy VO,Hååg P,Novak M,Végvári Á,Arapi V,Lewensohn R,Viktorsson K

37-4400 was used in Western Blot to elucidate the function of the Ephrin type-A receptor 2 on the DNA damage response pathway involved in resistance to ionizing radiation.

Sun Feb 28 00:00:00 UTC 2021

microRNA-451a promoter methylation regulated by DNMT3B expedites bladder cancer development via the EPHA2/PI3K/AKT axis.

BMC cancer

Liu B,Sun W,Gao W,Li L,Cao Z,Yang X,Liu J,Guo Y

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Western Blot

Wed Oct 21 00:00:00 UTC 2020

microRNA-451a promoter methylation regulated by DNMT3B expedites bladder cancer development via the EPHA2/PI3K/AKT axis.

BMC cancer

Liu B,Sun W,Gao W,Li L,Cao Z,Yang X,Liu J,Guo Y

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Western Blot

Wed Oct 21 00:00:00 UTC 2020

A novel immortalized hepatocyte-like cell line (imHC) supports in vitro liver stage development of the human malarial parasite Plasmodium vivax.

Malaria journal

Pewkliang Y,Rungin S,Lerdpanyangam K,Duangmanee A,Kanjanasirirat P,Suthivanich P,Sa-Ngiamsuntorn K,Borwornpinyo S,Sattabongkot J,Patrapuvich R,Hongeng S

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Immunofluorescence

Thu Jan 25 00:00:00 UTC 2018

Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis.

Nature communications

Lee H,Noh H,Mun J,Gu C,Sever S,Park S

37-4400 was used in immunocytochemistry describe the role of Anks1a in COPII-mediated EphA2 export from the endoplasmic reticulum

Tue Sep 13 00:00:00 UTC 2016

Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis.

Nature communications

Lee H,Noh H,Mun J,Gu C,Sever S,Park S

37-4400 was used in immunocytochemistry describe the role of Anks1a in COPII-mediated EphA2 export from the endoplasmic reticulum

Tue Sep 13 00:00:00 UTC 2016

Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer.

Oncotarget

Efazat G,Novak M,Kaminskyy VO,De Petris L,Kanter L,Juntti T,Bergman P,Zhivotovsky B,Lewensohn R,Hååg P,Viktorsson K

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Immunohistochemistry

Tue Sep 13 00:00:00 UTC 2016

Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer.

Oncotarget

Efazat G,Novak M,Kaminskyy VO,De Petris L,Kanter L,Juntti T,Bergman P,Zhivotovsky B,Lewensohn R,Hååg P,Viktorsson K

Published figure using EphA2 monoclonal antibody (Product # 37-4400) in Immunohistochemistry

Tue Sep 13 00:00:00 UTC 2016

Characterization of a novel angiogenic model based on stable, fluorescently labelled endothelial cell lines amenable to scale-up for high content screening.

Biology of the cell

Prigozhina NL,Heisel A,Wei K,Noberini R,Hunter EA,Calzolari D,Seldeen JR,Pasquale EB,Ruiz-Lozano P,Mercola M,Price JH

Sat Oct 01 00:00:00 UTC 2011

EphA2 immunoconjugate as molecularly targeted chemotherapy for ovarian carcinoma.

Journal of the National Cancer Institute

Lee JW,Han HD,Shahzad MM,Kim SW,Mangala LS,Nick AM,Lu C,Langley RR,Schmandt R,Kim HS,Mao S,Gooya J,Fazenbaker C,Jackson D,Tice DA,Landen CN,Coleman RL,Sood AK

37-4400 was used in immunohistochemistry and western blot to test if an anti-EphA2 monoclonal antibody linked to a chemotherapeutic agent has antitumor activity using ovarian cancer cell lines and tumor models

Wed Sep 02 00:00:00 UTC 2009

EphA2 immunoconjugate as molecularly targeted chemotherapy for ovarian carcinoma.

Journal of the National Cancer Institute

Lee JW,Han HD,Shahzad MM,Kim SW,Mangala LS,Nick AM,Lu C,Langley RR,Schmandt R,Kim HS,Mao S,Gooya J,Fazenbaker C,Jackson D,Tice DA,Landen CN,Coleman RL,Sood AK

Wed Sep 02 00:00:00 UTC 2009

A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo.

Cancer research

Jackson D,Gooya J,Mao S,Kinneer K,Xu L,Camara M,Fazenbaker C,Fleming R,Swamynathan S,Meyer D,Senter PD,Gao C,Wu H,Kinch M,Coats S,Kiener PA,Tice DA

Sat Nov 15 00:00:00 UTC 2008

Framework shuffling of antibodies to reduce immunogenicity and manipulate functional and biophysical properties.

Molecular immunology

Damschroder MM,Widjaja L,Gill PS,Krasnoperov V,Jiang W,Dall'Acqua WF,Wu H

37-4400 was used in western blot to characterize monoclonal antibodies that recognize EphA2 and EphB4

Sun Apr 01 00:00:00 UTC 2007

生物信息学

蛋白别名: ephrin receptor EphA2; Ephrin type-A receptor 2; Epithelial cell kinase; epithelial cell receptor protein tyrosine kinase; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK; Tyrosine-protein kinase receptor MPK-5; Tyrosine-protein kinase receptor SEK-2

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基因别名: ARCC2; AW545284; CTPA; CTPP1; CTRCT6; ECK; EPHA2; Myk2; Sek-2; Sek2

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UniProt ID: (Human) P29317, (Mouse) Q03145

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Entrez Gene ID: (Human) 1969, (Mouse) 13836, (Rat) 366492

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