Invitrogen

Epha1 Polyclonal Antibody

Epha1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Epha1 Antibody (PA5-47435) in IHC (F)

Immunohistochemical analysis of EphA1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-mouse EphA1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47435) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB (brown and counterstained with hematoxylin (blue). Specific labeling was localized to thymic stromal cells.

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产品信息

PA5-47435

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25 µg/mL

免疫组化（冰冻） (IHC (F))

5-15 µg/mL

流式细胞分析 (Flow)

2.5 µg per million cells

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse EphA1 Glu27-Glu548

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2607409

产品详细信息

In direct ELISAs, approximately 35% cross-reactivity with recombinant human EphA1 and less than 5% cross-reactivity with recombinant mouse (rm) EphA4, rmEphA6, and rmEphA7 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

EphA1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA1 is expressed in some human cancer cell lines and has been implicated in carcinogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。