Invitrogen

Estrogen Receptor Beta Monoclonal Antibody (PPZ0506)

Estrogen Receptor Beta Antibody in Western Blot (WB)

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Estrogen Receptor Beta Antibody (MA5-24807) in WB

Journal for immunotherapy of cancer 2021 - Figure 2 ERbeta signaling in CD8 + T cells confers tumor inhibition. (A) Schematic representation of the bone marrow adoptive transfer approach used to generate WT >WT or WT >KI mice. A 5-week-old CD45.1 + B6.SJL was irradiated (IR) and reconstituted with CD45.2 + WT or KI bone marrow. (B, C) B16 melanoma growth in WT >WT and KI >WT male (B) and female (C) chimera mice. (D) qRT-PCR for indicated genes from ... View More

Estrogen Receptor Beta Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

MA5-24807

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

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酶联免疫吸附实验 (ELISA)

0.2 µg/mL

免疫沉淀 (IP)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

PPZ0506

抗原

Estrogen Receptor beta antibody for 2-88 amino acid peptide in human ER beta. Ligand for estrogenic 17b-estradiol compound.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

physiological saline

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2717280

产品详细信息

This antibody specifically recognizes human ER beta but does not recognize human ER alpha.

靶标信息

Estrogen Receptor beta (ER beta, NR3A2) protein is approximately 55kD, though multiple isoforms ranging from 36-59kD have been described. Human ER beta was initially cloned and characterized from testis. Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Estrogen receptor beta (ER beta) binds estrogens with an affinity similar to that of estrogen receptor alpha. Some ER beta isoforms dominantly inhibit the activity of estrogen receptor alpha in reproductive tissues. ER beta is found widely in many tissues throughout the body and can act as a potent tumor suppressor, playing a crucial role in many cancer types such as prostate cancer, autism spectrum disorder, medullary thyroid carcinoma, De Quervain Disease and risk of cardiovascular disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Investigation of the Antitumor Effects of Tamoxifen and Its Ferrocene-Linked Derivatives on Pancreatic and Breast Cancer Cell Lines.

Pharmaceuticals (Basel, Switzerland)

Kalabay M,Szász Z,Láng O,Lajkó E,Pállinger É,Duró C,Jernei T,Csámpai A,Takács A,Kőhidai L

MA5-24807 was used in Immunohistochemistry to find two molecules possessing better cytotoxicity compared to their unmodified parent molecule while also being able to counter the negative effects of the presence of the GPER1 through the ER-independent mechanism of oxidative stress induction.

Sat Mar 05 00:00:00 UTC 2022

Estrogen receptor beta signaling in CD8⁺ T cells boosts T cell receptor activation and antitumor immunity through a phosphotyrosine switch.

Journal for immunotherapy of cancer

Yuan B,Clark CA,Wu B,Yang J,Drerup JM,Li T,Jin VX,Hu Y,Curiel TJ,Li R

MA5-24807 was used in Western Blot, Immunoprecipitation to support the development of ERβ agonists including S-equol in combination with ICB immunotherapy for cancer treatment.

Fri Jan 01 00:00:00 UTC 2021

Estrogen receptor beta signaling in CD8⁺ T cells boosts T cell receptor activation and antitumor immunity through a phosphotyrosine switch.

Journal for immunotherapy of cancer

Yuan B,Clark CA,Wu B,Yang J,Drerup JM,Li T,Jin VX,Hu Y,Curiel TJ,Li R

MA5-24807 was used in Western Blot, Immunoprecipitation to support the development of ERβ agonists including S-equol in combination with ICB immunotherapy for cancer treatment.

Fri Jan 01 00:00:00 UTC 2021

Estrogen receptor beta signaling in CD8⁺ T cells boosts T cell receptor activation and antitumor immunity through a phosphotyrosine switch.

Journal for immunotherapy of cancer

Yuan B,Clark CA,Wu B,Yang J,Drerup JM,Li T,Jin VX,Hu Y,Curiel TJ,Li R

MA5-24807 was used in Western Blot, Immunoprecipitation to support the development of ERβ agonists including S-equol in combination with ICB immunotherapy for cancer treatment.

Fri Jan 01 00:00:00 UTC 2021

Optimized immunohistochemical detection of estrogen receptor beta using two validated monoclonal antibodies confirms its expression in normal and malignant breast tissues.

Breast cancer research and treatment

Hawse JR,Carter JM,Aspros KGM,Bruinsma ES,Koepplin JW,Negron V,Subramaniam M,Ingle JN,Rech KL,Goetz MP

MA5-24807 was used in Immunohistochemistry to provide a reference for future studies aimed at determining the utility of ERβ as a prognostic and/or predictive biomarker in various tissues of benign or malignant states.

Wed Jan 01 00:00:00 UTC 2020

Interaction Proteomics Identifies ERbeta Association with Chromatin Repressive Complexes to Inhibit Cholesterol Biosynthesis and Exert An Oncosuppressive Role in Triple-negative Breast Cancer.

Molecular & cellular proteomics : MCP

Alexandrova E,Giurato G,Saggese P,Pecoraro G,Lamberti J,Ravo M,Rizzo F,Rocco D,Tarallo R,Nyman TA,Collina F,Cantile M,Di Bonito M,Botti G,Nassa G,Weisz A

MA5-24807 was used in Western Blotting to explore the potential of targeting Estrogen Receptor beta (ERβ) and its role in triple-negative breast cancer cells.

Sat Feb 01 00:00:00 UTC 2020

Small Non-Coding RNA Profiling Identifies miR-181a-5p as a Mediator of Estrogen Receptor Beta-Induced Inhibition of Cholesterol Biosynthesis in Triple-Negative Breast Cancer.

Cells

Alexandrova E,Lamberti J,Saggese P,Pecoraro G,Memoli D,Cappa VM,Ravo M,Iorio R,Tarallo R,Rizzo F,Collina F,Cantile M,Bonito MD,Botti G,Nassa G,Weisz A,Giurato G

MA5-24807 was used in Western Blotting to investigate microRNA-mediated regulation of gene expression in triple-negative breast cancer.

Fri Apr 03 00:00:00 UTC 2020

Knockdown of estrogen receptor β increases proliferation and affects the transcriptome of endometrial adenocarcinoma cells.

BMC cancer

Treeck O,Diepolder E,Skrzypczak M,Schüler-Toprak S,Ortmann O

MA5-24807 was used in Western Blotting to show siRNA knockdown of estrogen receptor β can enhance proliferation of endometrial cancer cell lines.

Mon Jul 29 00:00:00 UTC 2019

Knockdown of estrogen receptor β increases proliferation and affects the transcriptome of endometrial adenocarcinoma cells.

BMC cancer

Treeck O,Diepolder E,Skrzypczak M,Schüler-Toprak S,Ortmann O

MA5-24807 was used in Western Blotting to show siRNA knockdown of estrogen receptor β can enhance proliferation of endometrial cancer cell lines.

Mon Jul 29 00:00:00 UTC 2019

Differential estrogen-receptor activation regulates extracellular matrix deposition in human airway smooth muscle remodeling via NF-κB pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ambhore NS,Kalidhindi RSR,Pabelick CM,Hawse JR,Prakash YS,Sathish V

MA5-24807 was used in Western Blot to demonstrate differential ER signaling in controlling ECM production and deposition.

Sun Dec 01 00:00:00 UTC 2019

Estrogen receptor beta signaling inhibits PDGF induced human airway smooth muscle proliferation.

Molecular and cellular endocrinology

Ambhore NS,Katragadda R,Raju Kalidhindi RS,Thompson MA,Pabelick CM,Prakash YS,Sathish V

MA5-24807 was used in Western Blotting to evaluate the effects of selective ERα and ERβ modulators on platelet-derived growth factor (PDGF)-stimulated airway smooth muscle proliferation and the mechanisms involved.

Thu Nov 15 00:00:00 UTC 2018

生物信息学

蛋白别名: ER; ER beta; ER-beta; estrogen receptor 2 (ER beta); Estrogen receptor beta; estrogen receptor beta 4; Nuclear receptor subfamily 3 group A member 2

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基因别名: ER-BETA; Erb; ESR-BETA; ESR2; ESRB; ESTRB; NR3A2

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UniProt ID: (Human) Q92731

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Entrez Gene ID: (Human) 2100

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功能