Invitrogen

FAH Polyclonal Antibody

FAH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FAH Antibody (PA5-42049) in IHC (P)

Immunohistochemistry analysis of FAH was performed on fah-/- (left) and wildtype (right) mouse liver paraffin sections. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissue slides were ... View More

FAH Antibody in Immunohistochemistry (IHC)

Product Image Gallery

FAH Antibody — Antibody specificity was demonstrated by comparing wildtype and FAH deficient mouse liver. FAH signal is detected only in the wildtype mouse liver (right panel) in IHC using Anti - FAH polyclonal Antibody (Product # PA5-42049). {KD}

产品信息

PA5-42049

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:400

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Pig

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the N-terminal of human FAH (aa 2-51).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2609348

产品详细信息

Peptide sequence: SFIPVAEDSD FPIHNLPYGV FSTRGDPRPR IGVAIGDQIL DLSIIKHLFT

Sequence homology: Cow: 93%; Dog: 86%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Mouse: 93%; Pig: 93%; Rat: 93%

靶标信息

This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The compound heterozygous mutations of c.607G>a and c.657delC in the FAH gene are associated with renal damage with hereditary tyrosinemia type 1 (HT1).

Molecular genetics & genomic medicine

Chi H,Gan C,Jiang Y,Chen D,Qiu J,Yang Q,Chen Y,Wang M,Yang H,Jiang W,Li Q

PA5-42049 was used in Western Blotting to identify novel compound heterozygous mutations (NM_000137: c.657delC (p.K220Rfs*12) and c.607G>A (p.A203T)) in the fumarylacetoacetate hydrolase (FAH) gene in a family.

Sun Jan 01 00:00:00 UTC 2023

The compound heterozygous mutations of c.607G>a and c.657delC in the FAH gene are associated with renal damage with hereditary tyrosinemia type 1 (HT1).

Molecular genetics & genomic medicine

Chi H,Gan C,Jiang Y,Chen D,Qiu J,Yang Q,Chen Y,Wang M,Yang H,Jiang W,Li Q

Sun Jan 01 00:00:00 UTC 2023

Prolonged activity of the transposase helper may raise safety concerns during DNA transposon-based gene therapy.

Molecular therapy. Methods & clinical development

Imre G,Takács B,Czipa E,Drubi AB,Jaksa G,Latinovics D,Nagy A,Karkas R,Hudoba L,Vásárhelyi BM,Pankotai-Bodó G,Blastyák A,Hegedűs Z,Germán P,Bálint B,Ahmed Abdullah KS,Kopasz AG,Kovács A,Nagy LG,Sükösd F,Pintér L,Rülicke T,Barta E,Nagy I,Haracska L,Mátés L

PA5-42049 was used in Immunohistochemistry to reveal that the piggyBac transposase protein exhibits prolonged activity, which predicts the risk of oncogenesis by generating chromosomal double-strand breaks.

Thu Jun 08 00:00:00 UTC 2023

A versatile transposon-based technology to generate loss- and gain-of-function phenotypes in the mouse liver.

BMC biology

Kopasz AG,Pusztai DZ,Karkas R,Hudoba L,Abdullah KSA,Imre G,Pankotai-Bodó G,Migh E,Nagy A,Kriston A,Germán P,Drubi AB,Molnár A,Fekete I,Dani VÉ,Ocsovszki I,Puskás LG,Horváth P,Sükösd F,Mátés L

Published figure using FAH polyclonal antibody (Product # PA5-42049) in Immunohistochemistry

Fri Apr 01 00:00:00 UTC 2022

Genetically blocking HPD via CRISPR-Cas9 protects against lethal liver injury in a pig model of tyrosinemia type I.

Molecular therapy. Methods & clinical development

Gu P,Yang Q,Chen B,Bie YN,Liu W,Tian Y,Luo H,Xu T,Liang C,Ye X,Liu Y,Tang X,Gu W

PA5-42049 was used in Western Blot to report an improved single-step method to establish a biallelic (FAH-/- ) mutant porcine model using CRISPR-Cas9 and cytoplasmic microinjection.

Fri Jun 11 00:00:00 UTC 2021

Genetically blocking HPD via CRISPR-Cas9 protects against lethal liver injury in a pig model of tyrosinemia type I.

Molecular therapy. Methods & clinical development

Gu P,Yang Q,Chen B,Bie YN,Liu W,Tian Y,Luo H,Xu T,Liang C,Ye X,Liu Y,Tang X,Gu W

PA5-42049 was used in Western Blot to report an improved single-step method to establish a biallelic (FAH-/- ) mutant porcine model using CRISPR-Cas9 and cytoplasmic microinjection.

Fri Jun 11 00:00:00 UTC 2021

Genetically blocking HPD via CRISPR-Cas9 protects against lethal liver injury in a pig model of tyrosinemia type I.

Molecular therapy. Methods & clinical development

Gu P,Yang Q,Chen B,Bie YN,Liu W,Tian Y,Luo H,Xu T,Liang C,Ye X,Liu Y,Tang X,Gu W

PA5-42049 was used in Western Blot to report an improved single-step method to establish a biallelic (FAH-/- ) mutant porcine model using CRISPR-Cas9 and cytoplasmic microinjection.

Fri Jun 11 00:00:00 UTC 2021

Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.

Translational pediatrics

Ming G,Guo W,Cheng Y,Wang J

PA5-42049 was used in Western Blot to identify potential biomarkers from the serum samples of choledochal cyst patients for early diagnosis of the disease.