Invitrogen

FAS (CD95) Chimeric Recombinant Rabbit Monoclonal Antibody (R-125224)

FAS (CD95) Chimeric Antibody in Flow Cytometry (Flow)

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FAS (CD95) Chimeric Antibody (MA5-48080) in Flow

Flow Cytometry analysis of FAS (CD95) in Jurkat cells using FAS (CD95) Chimeric Monoclonal Antibody (Product # MA5-48080). Cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or FAS (CD95) Chimeric Monoclonal Antibody (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using ... View More

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产品信息

MA5-48080

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1:100

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫电镜技术 (IM)

Assay-dependent

T细胞活化 (TCA)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG, kappa

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

R-125224

抗原

Partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.02% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3074523

产品详细信息

Specificity: R-125224 binds to the extracellular portion of human Fas at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114).

Preparation: R-125224 is generated by the humanization of the murine HFE7A anti-Fas antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas or the L5178YA1 cell line expressing murine Fas, but not to the parental WR19L or L5178Y cells.

靶标信息

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

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基因别名: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

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UniProt ID: (Human) P25445

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Entrez Gene ID: (Human) 355

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功能

protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

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