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GALR1 Polyclonal Antibody

GALR1 Antibody in Immunocytochemistry (ICC/IF)

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GALR1 Antibody (AGR-011-200UL) in ICC/IF

Expression of Galanin receptor 1 in ratDRG - Immunocytochemical staining of GALR1 in a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. Paraformaldehyde-fixed and permeabilized DRG primary culture stained with Anti-GALR1 Antibody (#AGR-011), (1:100) followed by goat- Anti-rabbit-AlexaFluor-555 secondary Antibody (red). B, E. Nuclear fluorescence staining of cells using Hoechst 33342 (blue).C. Merged images of panels A and B.F. Merged images of panels D and E.

GALR1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

AGR-011-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200

免疫组化（冰冻） (IHC (F))

1:200

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

RSKPGKPRSTTN(C), corresponding to amino acid residues 60-71 of mouse Galanin receptor type 1, 1st extracellular loop

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

靶标信息

The neuropeptide galanin elicits a range of biological effects by interaction with specific G-protein-coupled receptors. Galanin receptors are seven-transmembrane proteins shown to activate a variety of intracellular second-messenger pathways. GALR1 inhibits adenylyl cyclase via a G protein of the Gi/Go family. GALR1 is widely expressed in the brain and spinal cord, as well as in peripheral sites such as the small intestine and heart.

仅用于科研。不用于诊断过程。未经明确授权不得转售。