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GATA1 Antibody (700727) in ICC/IF
Immunofluorescence analysis of Erythroid transcription factor was performed using log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GATA1 Recombinant Rabbit Monoclonal Antibody (4H15L5) (Product # 700727) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,... View More
产品信息
700727
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4H15L5
抗原
Recombinant protein corresponding to amino acids 1-200 of human GATA1.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532341
产品详细信息
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Eryf1; Erythroid transcription factor; erythroid transcription factor 1; GATA binding protein 1 (globin transcription factor 1); GATA-1; GATA-binding factor 1; GATA-binding protein 1; globin transcription factor 1; HGNC:4170; NF-E1 DNA-binding protein; nuclear factor, erythroid 1; transcription factor GATA1
基因别名: ERYF1; GATA-1; GATA1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT
UniProt ID: (Human) P15976, (Mouse) P17679
Entrez Gene ID: (Human) 2623, (Mouse) 14460
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
p53 binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
DNA binding, bending
chromatin DNA binding
sequence-specific DNA binding
C2H2 zinc finger domain binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
chromatin binding
metal ion binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription from RNA polymerase II promoter
cell-cell signaling
heart development
blood coagulation
negative regulation of cell proliferation
male gonad development
organ morphogenesis
tissue development
regulation of glycoprotein biosynthetic process
regulation of definitive erythrocyte differentiation
regulation of primitive erythrocyte differentiation
erythrocyte differentiation
megakaryocyte differentiation
platelet formation
basophil differentiation
eosinophil differentiation
negative regulation of bone mineralization
positive regulation of osteoblast proliferation
embryonic hemopoiesis
eosinophil fate commitment
negative regulation of apoptotic process
positive regulation of erythrocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
digestive tract development
erythrocyte development
homeostasis of number of cells within a tissue
positive regulation of peptidyl-tyrosine phosphorylation
platelet aggregation
transcriptional activation by promoter-enhancer looping
dendritic cell differentiation
cellular response to thyroid hormone stimulus
negative regulation of transcription regulatory region DNA binding
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
transcription, DNA-templated
regulation of transcription, DNA-templated
myeloid cell differentiation
cell development
