Invitrogen

GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377)

GLUT1 Antibody in Immunocytochemistry (ICC/IF)

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GLUT1 Antibody (MA5-31960) in ICC/IF

Immunofluorescence analysis of GLUT1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377) (Product # MA5-31960) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa ... View More

GLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GLUT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF7 cells were transfected with GLUT1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-GLUT1 Recombinant Rabbit Monoclonal Antibody (SA0377) (Product # MA5-31960). GLUT-1 shows a streak like pattern in cell lines as seen in all the 3 lanes. The siRNA Lane shows almost 90% loss of streak like signal. {KD}

产品信息

MA5-31960

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:50,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:5,000

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1:500

流式细胞分析 (Flow)

1:500-1:1,000

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SA0377

抗原

Synthetic peptide within Human GLUT1 aa 443-492

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809254

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease.

Nature communications

Schmidt S,Stautner C,Vu DT,Heinz A,Regensburger M,Karayel O,Trümbach D,Artati A,Kaltenhäuser S,Nassef MZ,Hembach S,Steinert L,Winner B,Jürgen W,Jastroch M,Luecken MD,Theis FJ,Westmeyer GG,Adamski J,Mann M,Hiller K,Giesert F,Vogt Weisenhorn DM,Wurst W

MA5-31960 was used in Flow Cytometry to demonstrate a correlation between OGDHC activity in our cellular model and the disease progression.

Thu Nov 23 00:00:00 UTC 2023

Sphingosine-1-phosphate-lyase deficiency affects glucose metabolism in a way that abets oncogenesis.

Molecular oncology

Afsar SY,Alam S,Fernandez Gonzalez C,van Echten-Deckert G

MA5-31960 was used in Western Blotting to report on a newly identified role of the S1P/S1PR1-3 axis in glucose metabolism in SGPL1-deficient mouse embryonic fibroblasts.

Sat Oct 01 00:00:00 UTC 2022

Sphingosine-1-phosphate-lyase deficiency affects glucose metabolism in a way that abets oncogenesis.

Molecular oncology

Afsar SY,Alam S,Fernandez Gonzalez C,van Echten-Deckert G

MA5-31960 was used in Western Blotting to report on a newly identified role of the S1P/S1PR1-3 axis in glucose metabolism in SGPL1-deficient mouse embryonic fibroblasts.

Sat Oct 01 00:00:00 UTC 2022

Activation of Wnt/β-catenin pathway mitigates blood-brain barrier dysfunction in Alzheimer's disease.

Brain : a journal of neurology

Wang Q,Huang X,Su Y,Yin G,Wang S,Yu B,Li H,Qi J,Chen H,Zeng W,Zhang K,Verkhratsky A,Niu J,Yi C

MA5-31960 was used in Immunohistochemistry-immunofluorescence to show dysfunctional blood-brain barrier in patients with Alzheimer's disease reflected by perivascular accumulation of blood-derived fibrinogen in the hippocampus and cortex, accompanied by decreased tight junction proteins Claudin-5 and glucose transporter Glut-1 in the brain endothelial cells.

Mon Dec 19 00:00:00 UTC 2022

Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target.

Nature communications

Cho H,Kwon HY,Sharma A,Lee SH,Liu X,Miyamoto N,Kim JJ,Im SH,Kang NY,Chang YT

MA5-31960 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot, Immunohistochemistry (Frozen) to demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

Mon Oct 10 00:00:00 UTC 2022

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.

Scientific reports

Risha Y,Minic Z,Ghobadloo SM,Berezovski MV

MA5-31960 was used in Flow cytometry/Cell sorting to demonstrate that exosomes are a rich source of breast cancer-related proteins and surface biomarkers that may be used for disease diagnosis and prognosis.

Tue Aug 11 00:00:00 UTC 2020

The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers.

Scientific reports

Risha Y,Minic Z,Ghobadloo SM,Berezovski MV

Tue Aug 11 00:00:00 UTC 2020

Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.

Cell reports

Wang W,Kini A,Wang Y,Liu T,Chen Y,Vukmanic E,Emery D,Liu Y,Lu X,Jin L,Lee SJ,Scott P,Liu X,Dean K,Lu Q,Fortuny E,James R,Kaplan HJ,Du J,Dean DC

MA5-31960 was used in Immunohistochemistry-immunofluorescence to provide evidence that contact between externalized phosphatidylserine (PS) on OS tips and apical RPE receptors activates Akt, linking phagocytosis with glucose transport to photoreceptors for new OS synthesis.

Tue Jul 30 00:00:00 UTC 2019

生物信息学

蛋白别名: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); glucose transporter glut1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1; HepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1

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基因别名: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1

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UniProt ID: (Human) P11166, (Mouse) P17809, (Rat) P11167

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Entrez Gene ID: (Human) 6513, (Mouse) 20525, (Rat) 24778

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