Invitrogen

GNAT2 Polyclonal Antibody

GNAT2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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GNAT2 Antibody (PA5-22340) in IHC (F)

Immunohistochemistry (Frozen) analysis of GNAT2 was performed in frozen sectioned adult mouse retina tissue using GNAT2 Polyclonal Antibody (Product # PA5-22340) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

GNAT2 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

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GNAT2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells and tissues owing to their inherent genetic constitution. Relative expression of GNAT2 was observed in Mouse Retina in comparison to Hep G2, HEK-293, A549, COLO205, Mouse Spleen, Mouse Brain and Mouse Heart using GNAT2 Polyclonal Antibody (Product # PA5-22340) in western blot. {RE}

产品信息

PA5-22340

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

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免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

1:100-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 154 and 354 of GNAT2 (Uniprot ID#P19087)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.26 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11153979

产品详细信息

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (87%), Xenopus laevis (91%), Chicken (94%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in cones.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A mouse model of cone photoreceptor function loss (cpfl9) with degeneration due to a mutation in Gucy2e.

Frontiers in molecular neuroscience

Naggert ASEN,Collin GB,Wang J,Krebs MP,Chang B

Published figure using GNAT2 polyclonal antibody (Product # PA5-22340) in Western Blot

Sat Nov 23 00:00:00 UTC 2024

BBSome Component BBS5 Is Required for Cone Photoreceptor Protein Trafficking and Outer Segment Maintenance.

Investigative ophthalmology & visual science

Bales KL,Bentley MR,Croyle MJ,Kesterson RA,Yoder BK,Gross AK

PA5-22340 was used in Immunohistochemistry (Frozen) to suggest that, although BBS5 is a core BBSome component expressed in all ciliated cells, its role within the retina mediates specific photoreceptor protein cargo transport.

Mon Aug 03 00:00:00 UTC 2020

BBSome Component BBS5 Is Required for Cone Photoreceptor Protein Trafficking and Outer Segment Maintenance.

Investigative ophthalmology & visual science

Bales KL,Bentley MR,Croyle MJ,Kesterson RA,Yoder BK,Gross AK

Mon Aug 03 00:00:00 UTC 2020

BBSome Component BBS5 Is Required for Cone Photoreceptor Protein Trafficking and Outer Segment Maintenance.

Investigative ophthalmology & visual science

Bales KL,Bentley MR,Croyle MJ,Kesterson RA,Yoder BK,Gross AK

Mon Aug 03 00:00:00 UTC 2020

Human L- and M-opsins restore M-cone function in a mouse model for human blue cone monochromacy.

Molecular vision

Deng WT,Li J,Zhu P,Chiodo VA,Smith WC,Freedman B,Baehr W,Pang J,Hauswirth WW

PA5-22340 was used in Immunohistochemistry to show that AAV5-mediated expression of either human M- or L-opsin individually or combined promotes regrowth of cone outer segments and rescues M-cone function in the treated Opn1mw-/- dorsal retina.

Mon Nov 12 00:00:00 UTC 2018

生物信息学

蛋白别名: cone-type transducin alpha subunit; guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 2; Guanine nucleotide-binding protein G(t) subunit alpha-2; RP5-1160K1.4; Transducin alpha-2 chain; transducin, cone-specific, alpha polypeptide

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基因别名: ACHM4; AW490837; Gnat-2; GNAT2; GNATC; Gt-2; Tcalpha

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UniProt ID: (Human) P19087, (Mouse) P50149

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Entrez Gene ID: (Human) 2780, (Mouse) 14686

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功能

G-protein coupled receptor binding GTPase activity signal transducer activity GTP binding G-protein coupled photoreceptor activity G-protein beta/gamma-subunit complex binding metal ion binding nucleotide binding guanyl nucleotide binding

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