Invitrogen

GSC Recombinant Rabbit Monoclonal Antibody (23GB6470)

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GSC Antibody (MA5-53962) in WB

Western Blot analysis of GSC. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GSC recombinant monoclonal antibody (Product # MA5-53962) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

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GSC Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Goosecoid homeobox shRNA and decrease in signal intensity was observed in Western Blotting using Anti-GSC Recombinant Rabbit Monoclonal Antibody (23GB6470) (Product # MA5-53962) at a dilution of 1:5,000. Secondary antibody used was a goat anti-rabbit (HRP) at a dilution of 1:20,000. {KD}

产品信息

MA5-53962

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB6470

抗原

A synthesized peptide derived from human GSC (1-37AA).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。