Invitrogen

HAI-1 Monoclonal Antibody (9B10), eBioscience™

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HAI-1 Antibody (14-9960-82) in Flow

Staining of MCF7 cell line with 0.25 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human HAI-1 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.

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产品信息

14-9960-82

应用

建议稀释比

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免疫印迹 (WB)

Assay-Dependent

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

9B10

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_891558

产品详细信息

Description: The monoclonal antibody 9B10 recognizes HAI-1 also known as hepatocyte growth factor activation inhibitor. HAI-1 is a single transmembrane protein of approximately 53 kDa with an extracellular Kunitz-type serine protease inhibitor domain. HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins. HAI can bind to and inhibit HGFA and matripase, which are responsible for converting HGF to an active form. Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate, small intestine, fetal kidney and fetal lung. HAI-1 is a marker for prostate and breast cancers.

Applications Reported: This 9B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB) under nonreducing conditions only.

Applications Tested: This 9B10 antibody has been tested by flow cytometric analysis of human cell line MCF7. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。