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HDAC2 Antibody (MA5-18061) in ICC/IF
Immunofluorescence analysis of HDAC2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HDAC2 Monoclonal Antibody (3F3) (Product MA5-18061) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175) at a dilution o... View More
产品信息
MA5-18061
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
3F3
抗原
Synthetic peptide, corresponding to amino acids 473-488 of Human HDAC2
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539444
产品详细信息
Western blot results show a band at 55 kDa, which matches the predicted molecular weight.
This antibody is predicted to detect HDAC2 in Chicken and Bovine samples.
靶标信息
HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HD2; hdac 2; hdac-2; Histone deacetylase 2; Protein deacylase HDAC2; transcriptional regulator homolog RPD3; YY1 transcription factor-binding protein; YY1-associated factor 1
基因别名: D10Wsu179e; HD2; HDAC2; mRPD3; RPD3; YAF1; Yy1bp
UniProt ID: (Human) Q92769, (Mouse) P70288
Entrez Gene ID: (Human) 3066, (Mouse) 15182, (Rat) 84577
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
core promoter binding
RNA polymerase II repressing transcription factor binding
chromatin binding
histone deacetylase activity
protein binding
transcription factor binding
deacetylase activity
enzyme binding
heat shock protein binding
nucleosomal DNA binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
protein deacetylase activity
sequence-specific DNA binding
poly(A) RNA binding
NF-kappaB binding
transcription factor activity, sequence-specific DNA binding
hydrolase activity
histone deacetylase activity (H3-K14 specific)
chromatin DNA binding
histone deacetylase activity (H3-K9 specific)
histone deacetylase activity (H4-K16 specific)
Krueppel-associated box domain binding
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
NAD-dependent histone deacetylase activity (H3-K18 specific)
negative regulation of transcription from RNA polymerase II promoter
response to amphetamine
cardiac muscle hypertrophy
chromatin remodeling
maintenance of chromatin silencing
transcription, DNA-templated
blood coagulation
positive regulation of cell proliferation
epidermal cell differentiation
positive regulation of epithelial to mesenchymal transition
positive regulation of receptor biosynthetic process
negative regulation of neuron projection development
dendrite development
histone deacetylation
response to caffeine
response to lipopolysaccharide
positive regulation of interleukin-1 production
positive regulation of tumor necrosis factor production
circadian regulation of gene expression
positive regulation of collagen biosynthetic process
cellular response to heat
response to nicotine
response to cocaine
odontogenesis of dentin-containing tooth
response to drug
positive regulation of tyrosine phosphorylation of Stat3 protein
embryonic digit morphogenesis
ATP-dependent chromatin remodeling
negative regulation of apoptotic process
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of MHC class II biosynthetic process
positive regulation of proteolysis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
behavioral response to ethanol
positive regulation of oligodendrocyte differentiation
response to hyperoxia
hair follicle placode formation
negative regulation of dendritic spine development
eyelid development in camera-type eye
fungiform papilla formation
cellular response to hydrogen peroxide
histone H3 deacetylation
histone H4 deacetylation
cellular response to retinoic acid
cellular response to transforming growth factor beta stimulus
regulation of signal transduction by p53 class mediator
cellular response to dopamine
negative regulation of peptidyl-lysine acetylation
regulation of transcription, DNA-templated
protein deacetylation
chromatin modification
hippocampus development
neuron differentiation
rhythmic process
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of protein kinase B signaling
cardiac muscle cell development
negative regulation of cardiac muscle cell proliferation
regulation of sarcomere organization
negative regulation of canonical Wnt signaling pathway
regulation of protein deacetylation
negative regulation of intrinsic apoptotic signaling pathway
histone H3-K9 modification
