Invitrogen

HGF Monoclonal Antibody (SBF5)

HGF Antibody in Immunocytochemistry (ICC/IF)

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HGF Antibody (MA5-14160) in ICC/IF

Immunofluorescent analysis of Hepatocyte Growth Factor (green) showing staining in the cytoplasm of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Hepatocyte Growth Factor monoclonal antibody (Product # MA5-14160) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS... View More

HGF Antibody in Immunohistochemistry (IHC)

Product Image Gallery

HGF Antibody — Figure 1 Increased expression of HGF in the nerve injury site. (A) Time kinetics of HGF expression in the sciatic nerve after nerve injury. Following nerve crush, total ipsilateral sciatic nerves were isolated at appropriate time points, and total proteins were analyzed by ELISA to measure the HGF protein level. (B) Spatial distribution of HGF expression in the injured sciatic nerve. The level of the HGF protein was measured in three different areas of the sciatic nerve (proximal, injury and distal sites) at 4 d.p.i by ELISA. ***p < 0.001, ****p < 0.0001, n.s = not significant (C) High level expression of HGF in the injured site of sciatic nerve. Ipsilateral nerves were isolated at 4 d.p.i and subjected to immunohistochemical assay using an antibody to HGF (red). Scale bar = 100 µm (D) HGF expression in DRG and thigh muscles detected at 4 d.p.i. The protein level of HGF was measured in thigh muscles at 0, and 4 d.p.i by ELISA. n = 3 for each group. n.s = not significant. Values represent the mean S.E.M. {TM}

产品信息

MA5-14160

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:20-1:200

免疫电泳 (IE)

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

SBF5

抗原

Recombinant human HGF protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_10979474

产品详细信息

MA5-14160 targets Hepatocyte Growth Factor in WB and ICC/IF applications and shows reactivity with Human and Mouse samples.

The MA5-14160 immunogen is recombinant human HGF protein.

靶标信息

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer.

Cancers

Bauman JE,Ohr J,Gooding WE,Ferris RL,Duvvuri U,Kim S,Johnson JT,Soloff AC,Wallweber G,Winslow J,Gaither-Davis A,Grandis JR,Stabile LP

MA5-14160 was used in Immunoelectrophoresis to establish the recommended Phase II dose (RP2D). Secondary objectives included overall response rate (ORR), progression-free survival (PFS), and overall survival (OS).

Thu Jun 11 00:00:00 UTC 2020

Hepatocyte Growth Factor (HGF) Promotes Peripheral Nerve Regeneration by Activating Repair Schwann Cells.

Scientific reports

Ko KR,Lee J,Lee D,Nho B,Kim S

Published figure using HGF monoclonal antibody (Product # MA5-14160) in Immunofluorescence

Tue May 29 00:00:00 UTC 2018

Resistance to targeted cancer drugs through hepatocyte growth factor signaling.

Cell cycle (Georgetown, Tex.)

Heynen GJ,Fonfara A,Bernards R

MA5-14160 was used in western blot to study hepatocyte growth factor signaling and resistance to targeted cancer drugs

Fri Sep 04 00:00:00 UTC 2015

Hepatocyte growth factor in hepatic allograft biopsies: an immunohistochemical study.

Transplantation proceedings

Bilezikçi B,Demirhan B,Emiroğlu R,Haberal M

MA5-14160 was used in immunohistochemistry to study the association of hepatocyte growth factor induction with acute rejection in liver transplants

Mon Dec 01 00:00:00 UTC 2003

生物信息学

蛋白别名: Fibroblast derived tumor cytotoxic factor; fibroblast-derived tumor cytotoxic factor; Hepatocyte growth factor; Hepatocyte growth factor (hepapoietin A; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain; Hepatopoeitin A; Hepatopoietin-A; HepatopoietinA; Lung fibroblast derived mitogen; lung fibroblast-derived mitogen; Scatter factor; scatter factor); SF

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基因别名: C230052L06Rik; DFNB39; F-TCF; HGF; HGF/SF; HGFB; HPTA; NK1; NK2; SF; SF/HGF

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UniProt ID: (Human) P14210, (Mouse) Q08048

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Entrez Gene ID: (Human) 3082, (Mouse) 15234

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功能

serine-type endopeptidase activity protein binding growth factor activity chemoattractant activity protein heterodimerization activity identical protein binding

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