Invitrogen

HO-1 Polyclonal Antibody

图: 1 / 11

HO-1 Antibody (PA5-77833) in WB

Western blot was performed using HO-1 Polyclonal Antibody (Product # PA5-77833), and a 32 kDa band corresponding to HO-1 protein was observed across cell lines and tissues tested except MCF-7 which is reported to have low to negative expression. An increase in HO-1 expression was observed in HeLa cells on cobalt chloride treatment. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Cobalt Chloride (500 µM for 24 hours) (Lane 2), PANC-1 (Lane 3), MIA PaCa-2 (Lane 4), K-562 (Lane 5), A549 (Lane 6), MCF7 (Lane 7), and tiss... View More

HO-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

HO-1 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using HO-1 Polyclonal Antibody (Product # PA5-77833) shows up-regulation of HO-1 protein in HeLa cells upon cobalt chloride treatment. {TM}

产品信息

PA5-77833

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看7篇已发表文章 7篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:100

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Dog, Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide (aa. 1-30)1 : MERPQPDSMPQDLSEALKEATKEVHTQAEN

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2735912

产品详细信息

1 µg/mL of PA5-77833 was sufficient for detection of HO-1 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 32kDa.

靶标信息

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Protective Effect of Fucoxanthin on Zearalenone-Induced Hepatic Damage through Nrf2 Mediated by PI3K/AKT Signaling.

Marine drugs

Ben Ammar R,Zahra HA,Abu Zahra AM,Alfwuaires M,Abdulaziz Alamer S,Metwally AM,Althnaian TA,Al-Ramadan SY

Published figure using HO-1 polyclonal antibody (Product # PA5-77833) in Western Blot

Mon Jul 03 00:00:00 UTC 2023

Protective effects of aloin on asthmatic mice by activating Nrf2/HO-1 pathway and inhibiting TGF-β/Smad2/3 pathway.

Allergologia et immunopathologia

Wu S,Xia Y,Yang C,Li M

PA5-77833 was used in Western Blot to investigate the effects and mechanism of aloin on the ovalbumin-treated mice.

Tue Jul 11 00:00:00 UTC 2023

Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

Translational andrology and urology

Su Y,Xu J,Chen S,Feng J,Li J,Lei Z,Qiao L,Wang Y,Zeng D

PA5-77833 was used in Immunohistochemistry and Western Blotting and Immunohistochemistry to investigate the protective effects of Astragaloside IV (AS-IV) on ischemia-reperfusion (I/R) renal injury based on the keap1-Nrf2/ARE signaling pathway.

Mon Aug 01 00:00:00 UTC 2022

Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

Translational andrology and urology

Su Y,Xu J,Chen S,Feng J,Li J,Lei Z,Qiao L,Wang Y,Zeng D

Mon Aug 01 00:00:00 UTC 2022

Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

Translational andrology and urology

Su Y,Xu J,Chen S,Feng J,Li J,Lei Z,Qiao L,Wang Y,Zeng D

Mon Aug 01 00:00:00 UTC 2022

Ruscogenin Protects Against Deoxynivalenol-Induced Hepatic Injury by Inhibiting Oxidative Stress, Inflammation, and Apoptosis Through the Nrf2 Signaling Pathway: An In vitro Study.

Saudi journal of medicine & medical sciences

Elsawy H,Rajendran P,Sedky AM,Alfwuaires M

Published figure using HO-1 polyclonal antibody (Product # PA5-77833) in Western Blot

Wed Oct 19 00:00:00 UTC 2022

Flavonoid-Conjugated Gadolinium Complexes as Anti-Inflammatory Theranostic Agents.

Antioxidants (Basel, Switzerland)

Yang BW,Yang S,Kim S,Baek AR,Sung B,Kim YH,Lee JT,Lee SY,Kim HK,Choi G,Park JA,Nam SW,Lee GH,Chang Y

Published figure using HO-1 polyclonal antibody (Product # PA5-77833) in Western Blot

Thu Dec 15 00:00:00 UTC 2022

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

International journal of molecular sciences

Alzahrani AM,Rajendran P,Veeraraghavan VP,Hanieh H

Published figure using HO-1 polyclonal antibody (Product # PA5-77833) in Western Blot

Tue Mar 23 00:00:00 UTC 2021

Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies.

International journal of molecular sciences

Rajendran P,Ammar RB,Al-Saeedi FJ,Mohamed ME,ElNaggar MA,Al-Ramadan SY,Bekhet GM,Soliman AM

PA5-77833 was used in Western Blotting to demonstrate that kaempferol possesses notable hepatoprotective roles against zearalenone-induced damage, potentially through the stimulation of Nrf2/HO-1 cascades and PI3K/Akt signalling.

Mon Dec 28 00:00:00 UTC 2020

Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies.

International journal of molecular sciences

Rajendran P,Ammar RB,Al-Saeedi FJ,Mohamed ME,ElNaggar MA,Al-Ramadan SY,Bekhet GM,Soliman AM

Mon Dec 28 00:00:00 UTC 2020

生物信息学

蛋白别名: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein

查看更多收起

基因别名: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

查看更多收起

UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901

查看更多收起

Entrez Gene ID: (Human) 3162, (Dog) 442987, (Rat) 24451, (Mouse) 15368

查看更多收起

功能