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HRD1 Antibody (PA5-100081) in ICC/IF
Immunofluorescent analysis of HRD1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and HRD1 polyclonal antibody (Product # PA5-100081) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100081
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SYVN1(Accession Q86TM6), corresponding to amino acid residues P348-P398.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2815611
产品详细信息
Antibody detects endogenous levels of total SYVN1.
靶标信息
HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. It has been reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its anti-apoptotic effects. HRD1 contains one ring-type zinc finger.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog; HRD1 protein; MGC40372; RING-type E3 ubiquitin transferase synoviolin; Synovial apoptosis inhibitor 1; synovial apoptosis inhibitor 1, synoviolin; synoviolin 1
基因别名: 1200010C09Rik; AW211966; C85322; D530017H19Rik; DER3; HRD1; KIAA1810; RGD1310488; SYVN1
UniProt ID: (Human) Q86TM6, (Mouse) Q9DBY1
Entrez Gene ID: (Human) 84447, (Mouse) 74126, (Rat) 361712
protein binding
zinc ion binding
ligase activity
unfolded protein binding
chaperone binding
ATPase binding
ubiquitin protein ligase activity
ubiquitin protein ligase activity involved in ERAD pathway
ubiquitin-specific protease binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
activating transcription factor binding
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
acid-amino acid ligase activity
in utero embryonic development
response to unfolded protein
multicellular organism development
protein ubiquitination
protein N-linked glycosylation via asparagine
protein catabolic process
ER-associated ubiquitin-dependent protein catabolic process
retrograde protein transport, ER to cytosol
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
endoplasmic reticulum unfolded protein response
IRE1-mediated unfolded protein response
ERAD pathway
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
protein stabilization
protein K48-linked ubiquitination
endoplasmic reticulum mannose trimming
