Rockland

Hexokinase Polyclonal Antibody, HRP

Hexokinase Antibody in Western Blot (WB)

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Hexokinase Antibody (200-4359-0100) in WB

Western Blot of Rabbit anti-Hexokinase Antibody Peroxidase Conjugated. Lane 1: Hexokinase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Hexokinase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a Block: MB-070 for 30 min at RT. Predicted/Observed size: 54 kDa, 54 kDa for Hexokinase.

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产品信息

200-4359-0100

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

酶联免疫吸附实验 (ELISA)

1:1,000-1:2,500

免疫沉淀 (IP)

1:500-1:1,000

产品规格

种属反应

Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Hexokinase [Yeast]

偶联物

HRP HRP HRP

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Ion-exchange chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% gentamicin sulfate

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-HEXOKINASE (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast]. Cross reactivity against Hexokinase from other tissues and species is expected based on sequence homology.

靶标信息

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。