Invitrogen

Human Serum Albumin Recombinant Rabbit Monoclonal Antibody (101)

Human Serum Albumin Antibody in Immunocytochemistry (ICC/IF)

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Human Serum Albumin Antibody (MA5-29022) in ICC/IF

Immunofluorescence staining of Human HSA in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Human Serum Albumin Recombinant Rabbit Monoclonal Antibody (101) (Product # MA5-29022, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining... View More

Human Serum Albumin Antibody in Western Blot (WB)

Human Serum Albumin Antibody in Flow Cytometry (Flow)

Human Serum Albumin Antibody in Immunoprecipitation (IP)

Human Serum Albumin Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

MA5-29022

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:20-1:100

流式细胞分析 (Flow)

1:25-1:100

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酶联免疫吸附实验 (ELISA)

1:5,000-1:10,000

免疫沉淀 (IP)

0.5-2 µL/mg of lysate

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

101

抗原

Human Serum Albumin/HSA

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 6

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2784959

产品详细信息

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

This antibody has specificity for Human Serum Albumin/HSA.

靶标信息

Albumin is a soluble, monomeric protein with a molecular weight of approximately 65-67 kDa that comprises about one-half of the blood serum protein. Human serum albumin (HAS) is the most abundant protein in human blood plasma, and has a serum half-life of approximately 20 days. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular, unglycosylated serum protein synthesized in the liver as pre-pro-albumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough `produce the secreted albumin. Serum albumin has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. The main function of albumin is the regulation of the colloidal osmotic pressure of blood.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Programmable half-life and anti-tumour effects of bispecific T-cell engager-albumin fusions with tuned FcRn affinity.

Communications biology

Mandrup OA,Ong SC,Lykkemark S,Dinesen A,Rudnik-Jansen I,Dagnæs-Hansen NF,Andersen JT,Alvarez-Vallina L,Howard KA

MA5-29022 was used in Flow cytometry/Cell sorting to demonstrate in a humanised FcRn/albumin double transgenic mouse model (AlbuMus) the ability to tune half-life based on the albumin sequence fused with a BiTE-like bispecific (anti-EGFR nanobody x anti-CD3 scFv) light T-cell engager (LiTE) construct [(t½ 0.6 h (Fc-less LiTE), t½ 19 hours (Albu-LiTE-NB), t½ 26 hours (Albu-LiTE-WT), t½ 37 hours (Albu-LiTE-HB)].