Invitrogen

ID4 Polyclonal Antibody

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5 篇参考文献

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抗体检测数据 (3)
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ID4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ID4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ID4 Antibody (PA5-26976) in IHC (P)

Immunohistochemistry analysis of ID4 in formalin fixed and paraffin embedded bladder carcinoma. Samples were incubated with ID4 polyclonal antibody (Product # PA5-26976) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ID4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ID4 Antibody in Western Blot (WB)

ID4 Antibody in Flow Cytometry (Flow)

ID4 Antibody in Immunocytochemistry (ICC/IF)

ID4 Antibody in Immunocytochemistry (ICC/IF)

ID4 Antibody in Immunohistochemistry (IHC)

ID4 Antibody in Immunohistochemistry (IHC)

ID4 Antibody in Flow Cytometry (Flow)

ID4 Antibody in Flow Cytometry (Flow)

Product Image Gallery

ID4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ID4 in formalin fixed and paraffin embedded bladder carcinoma. Samples were incubated with ID4 polyclonal antibody (Product # PA5-26976) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ID4 Antibody in Western Blot (WB) — Western blot analysis of ID4 in mouse bladder tissue lysates. Samples were incubated with ID4 polyclonal antibody (Product # PA5-26976). Lysates: 35 µg/lane. ID4 (arrow).

ID4 Antibody in Flow Cytometry (Flow) — Flow cytometry of ID4 in 293 cells (right histogram). Samples were incubated with ID4 polyclonal antibody (Product # PA5-26976) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control (left histogram).

ID4 Antibody in Immunocytochemistry (ICC/IF) — 3 A: Immunocytochemistry (ICC) of MVH and ID4 as proliferation-related markers in spermatogonial cells (SCs) cocultured with Leydig cell to evaluate to role for osteocalcin. The markers were stained green, and nuclei stained red in the cells (Scale bars = 100 mum). B,C: Quantification of the ICC data showed no significant expressions for MVH and ID4 in the osteocalcin-treated cells. Control, a-a'''', c-c'''', e-e''', and g-g'''; and osteocalcin, b-b'''', d-d'''', f-f''', and h-h'''

ID4 Antibody in Immunocytochemistry (ICC/IF) — 3 Immunofluorescence staining of spermatogonial cells (SCs) morphology for identifying proliferative (MVH and ID4) markers assessed at the end of weeks 1 and 2. Green stained SCs shows expression of the markers in the cells. Nuclei were stained red in the cells (Scale bars = 50 mum). Graphs are indicative of the quantified area stained fluorescently for the markers. a-a'''' e-e'''' i-i'''' m-m'''' control; b-b'''' f-f'''' j-j'''' n-n'''' Kp 10 -8 M; c-c'''' g-g'''' k-k'''' o-o'''' Kp 10 -7 M; and d-d'''' h-h'''' l-l'''' p-p'''' Kp 10 -6 M. a = P <= 0.001 vs Kp 10 -8 M + Kp 10 -7 M; b = P <= 0.03 vs control + Kp 10 -8 M; c = P <= 0.04 vs control + Kp 10 -8 M; d = P <= 0.02 vs control + Kp 10 -8 M; e = P <= 0.01 vs Kp 10 -8 muM. Kp, Kisspeptin

ID4 Antibody in Immunohistochemistry (IHC) — Figure 3 Immunohistochemistry staining for SCP3 protein; A: Control (with normal expression of ID4 protein in the seminiferous tubule), B: T1 (torsion-detorsion + single dose 100 mg/kg PTX with significant increase in the expression of scp3 protein (Yellow arrow)), C: T2 (torsion-detorsion + 25 mg/kg/day PTX for two weeks with significant increase in expression of sp3 protein, D: T/D (torsion-detorsion without any treatment and with significantly low expression of scp3 protein. (H & E, scale bar: 20 mum). (Full, non-unadjusted images are shown as supplementary material). Figure 3

ID4 Antibody in Immunohistochemistry (IHC) — Figure 2 Immunohistochemistry staining for ID4 protein: A: Control with normal expression of ID4 protein in the seminiferous tubule, B: T1 (torsion-detorsion + s ingle dose of 100 mg/kg PTX with significant expression of ID4 protein (yellow arrow)), C: T2 (torsion-detorsion + 25 mg/kg/day PTX for two weeks with low expression of ID4 protein, D: T/D (torsion-detorsion without any treatment and with significantly low expression of ID4 protein. (H & E, scale bar: 20 mum). (Full, non-unadjusted images are shown as supplementary material). Figure 2

ID4 Antibody in Flow Cytometry (Flow) — 1 A, Morphological and identity of spermatogonial cells (SCs). Large and dense colonies are visible in an image taken by an inverted microscope at the end of the first week of culture (a'''). Results from alkaline phosphatase staining indicate apparent cellular colonies after culturing for 14 (a'''') and 28 (a'''''') days (Scale bar = 100 mum). a', day 1; a'', day 4; and; a''''', day 21. B, ID4, alpha6-, and beta1-integrin were assessed by the flow cytometry to determine whether the isolated cells had SC identity. As it is evident, these markers were expressed at high percentages in the cells. b' and b'', control; b''', ID4; b'''', alpha6-integrin; and b''''', beta1-integrin

ID4 Antibody in Flow Cytometry (Flow) — 1 Spermatogonial cell (SC) morphology and identity. SCs growing in the culture showed large and compact colonies by the end of the first week. a', day 1; a'', day 4; a''', day 7; a'''', day 14; a''''', day 21; and a'''''', day 28. a''''''' shows red staining colonies of SCs after alkaline phosphatase staining (scale bars = 100 mum). B, flow cytometry analysis for SC markers ID4 (b'), alpha6 integrin (b'') and beta1 integrin (b'''). Negative controls for FITC and PE were marker by respective b'''' and b'''''. PE, phycoerythrin

产品信息

PA5-26976

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:100

-

免疫细胞化学 (ICC/IF)

-

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

1:10-1:50

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ID4

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2544476

产品详细信息

This antibody is predicted to react with porcine based on sequence homology.

靶标信息

ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pentoxifylline improves the survival of spermatogenic cells via oxidative stress suppression and upregulation of PI3K/AKT pathway in mouse model of testicular torsion-detorsion.

Heliyon

Dhulqarnain AO,Takzaree N,Hassanzadeh G,Tooli H,Malekzadeh M,Khanmohammadi N,Yaghobinejad M,Solhjoo S,Rastegar T

Published figure using ID4 polyclonal antibody (Product # PA5-26976) in Immunohistochemistry

Thu Apr 01 00:00:00 UTC 2021

Roles for osteocalcin in proliferation and differentiation of spermatogonial cells cocultured with somatic cells.

Journal of cellular biochemistry

Solhjoo S,Akbari M,Toolee H,Mortezaee K,Mohammadipour M,Nematollahi-Mahani SN,Shahrokhi A,Sayadi M,Rastegar T

PA5-26976 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to show that the SCs were the population of cells able to form colonies and to express ID4, a6-, and ß1-integrin markers, respectively.

Mon Apr 01 00:00:00 UTC 2019

Roles for osteocalcin in proliferation and differentiation of spermatogonial cells cocultured with somatic cells.

Journal of cellular biochemistry

Solhjoo S,Akbari M,Toolee H,Mortezaee K,Mohammadipour M,Nematollahi-Mahani SN,Shahrokhi A,Sayadi M,Rastegar T

PA5-26976 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to show that the SCs were the population of cells able to form colonies and to express ID4, a6-, and ß1-integrin markers, respectively.

Mon Apr 01 00:00:00 UTC 2019

Roles for osteocalcin in proliferation and differentiation of spermatogonial cells cocultured with somatic cells.

Journal of cellular biochemistry

Solhjoo S,Akbari M,Toolee H,Mortezaee K,Mohammadipour M,Nematollahi-Mahani SN,Shahrokhi A,Sayadi M,Rastegar T

PA5-26976 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to show that the SCs were the population of cells able to form colonies and to express ID4, a6-, and ß1-integrin markers, respectively.

Mon Apr 01 00:00:00 UTC 2019

Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells.

Journal of cellular biochemistry

Toolee H,Rastegar T,Solhjoo S,Mortezaee K,Mohammadipour M,Kashani IR,Akbari M

PA5-26976 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to conclude that Kp as a neuropeptide for influencing central part of reproductive axis could also positively affect peripheral processes related to spermatogenesis without having an effect on steroidogenesis.

Mon Apr 01 00:00:00 UTC 2019

Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells.

Journal of cellular biochemistry

Toolee H,Rastegar T,Solhjoo S,Mortezaee K,Mohammadipour M,Kashani IR,Akbari M

PA5-26976 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to conclude that Kp as a neuropeptide for influencing central part of reproductive axis could also positively affect peripheral processes related to spermatogenesis without having an effect on steroidogenesis.

Mon Apr 01 00:00:00 UTC 2019

Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells.

Journal of cellular biochemistry

Toolee H,Rastegar T,Solhjoo S,Mortezaee K,Mohammadipour M,Kashani IR,Akbari M

PA5-26976 was used in Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot to conclude that Kp as a neuropeptide for influencing central part of reproductive axis could also positively affect peripheral processes related to spermatogenesis without having an effect on steroidogenesis.

Mon Apr 01 00:00:00 UTC 2019

生物信息学

蛋白别名: bHLHb27; Class B basic helix-loop-helix protein 27; DNA-binding protein inhibitor ID-4; Inhibitor of differentiation 4; Inhibitor of DNA binding 4; inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

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基因别名: BHLHB27; Id-4; ID4; IDB4

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UniProt ID: (Human) P47928, (Mouse) P41139

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Entrez Gene ID: (Human) 3400, (Mouse) 15904

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RNA polymerase II transcription factor binding transcription corepressor activity protein binding protein dimerization activity

G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription from RNA polymerase II promoter neuroblast proliferation circadian rhythm positive regulation of cell proliferation hippocampus development cerebral cortex neuron differentiation central nervous system myelination cellular protein localization fat cell differentiation negative regulation of fat cell differentiation negative regulation of neuron differentiation positive regulation of osteoblast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation prostate gland epithelium morphogenesis prostate gland stromal morphogenesis seminal vesicle morphogenesis regulation of transcription, DNA-templated brain development cell proliferation