Invitrogen

IL-17F Monoclonal Antibody (H17F10A7), eBioscience™

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IL-17F Antibody (14-7479-82) in WB

IL-17 producing cells were generated by culturing human PBMC with anti-Human CD3 (Product # 16-0039-81) and anti-Human CD28 (Product # 16-0289-81) in the presence of Human IL-23 Recombinant for 3 days, followed by 3 days with IL-23 alone. Day 6 cultures were treated with Brefeldin A alone (1) or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours. Cell lysates were run under reducing conditions, immunoblotted with 2 µg/mL of Anti-Human IL-17F Purified and revealed with Anti-Mouse HRP.

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产品信息

14-7479-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

流式细胞分析 (Flow)

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产品规格

种属反应

Human

已发表种属

Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

H17F10A7

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_891574

产品详细信息

Description: The monoclonal antibody H17F10A7 reacts with human IL-17F, a member of the IL-17 family of pro-inflammatory cytokines. Like IL-17A, IL-17F is a disulfide-linked, homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-β, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-β and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). These heterodimers can be detected by immunoprecipitation with eBio64CAP17 anti-IL-17A monoclonal antibody followed by immunoblot with H17F10A7.

Applications Reported: This H17F10A7 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This H17F10A7 antibody has been tested at 1-2µg/mL by western blot analysis of cultured, activated Th17 cells, where it detects a band of approximately 18kDa.

It is recommended that H17F10A7 be used under reducing conditions only.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflammation by increasing chemokine production. IL-17F also stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17F is produced, and exists, as a homo-dimer, with homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells. IL-17F has been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

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