Search Thermo Fisher Scientific
Invitrogen
Description: This AB12-3G4 monoclonal antibody reacts with and inhibits the bioactivity of human IL-2. IL-2 is a 15 kDa cytokine expressed by B cells, T cells, and dendritic cells. IL-2 signals via a heterotrimeric receptor (CD25) and results in the activation of Jak/STAT pathways. IL-2 plays a critical role in the proliferation, activation, and differentiation of T cells. The development of CD4+CD25+ regulatory T cells is especially dependent on the presence of IL-2, making it an important immunoregulatory cytokine.
Applications Reported: This AB12-3G4 monoclonal antibody inhibits the bioactivity of human IL-2.
Applications Tested: The ND50 of AB12-3G4, as determined by the neutralization of Human IL-2-induced proliferation of the CTLL-2 cell line, is less than or equal to 60 ng/mL in the presence of 10 ng/mL Human IL-2 Recombinant Protein. The neutralization dose of this antibody will vary depending on cell type, assay method, and cytokine concentration.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF
基因别名: IL-2; IL2; lymphokine; TCGF
UniProt ID: (Human) P60568
Entrez Gene ID: (Human) 3558