Invitrogen

IL-20 Polyclonal Antibody

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IL-20 Antibody (PA5-47067) in Neu

Neutralization of IL-20 in BaF3 mouse pro-B cell line co-transfected with human IL-20 Rα and IL-20 Rβ. Samples were incubated in IL-20 polyclonal antibody (Product # PA5-47067). Recombinant Human IL-20 stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Rα and IL-20 Rβ in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-20 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-20 Antigen Affinity-purified Polyclonal Antibody. The N... View More

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IL-20 Antibody — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL-20 is shown by the decrease in mean RFU (relative fluorescence units – measure of cell proliferation) with increasing concentrations of IL-20 polyclonal antibody (Product # PA5-47067). {Neu}

产品信息

PA5-47067

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

中和实验 (Neu)

0.1-0.4 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant human IL-20 Leu25-Glu176

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2576908

产品详细信息

In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) IL-19 is observed, approximately 15% cross-reactivity with recombinant mouse IL-20 is observed, and less than 1% cross-reactivity with rhIL-10 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

Cytokines that signal through Class II receptors form a distinct family that includes the interferons and interleukin 10 (IL-10). Recent identification of several IL-10 homologs has defined a cytokine subfamily that includes AK155, IL-19, IL-20, IL-22, and IL-24. IL-20 belongs to the IL-10 family and plays a role in skin inflammation and the development of hematopoietic cells. Little is known about its other biological functions and clinical implications. These cytokines display pro-inflammatory activities in different tissues, including skin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。